Complete degradation of tetrachloroethene in coupled anoxic and oxic chemostats

Applied Microbiology and Biotechnology
J GerritseJ C Gottschal

Abstract

Anaerobic tetrachloroethene (C2Cl4)-dechlorinating bacteria were enriched in slurries from chloroethene-contaminated soil. With methanol as electron donor, C2Cl4 and trichloroethene (C2HCl3) were reductively dechlorinated to cis-1,2-dichloroethene (cis-C2H2Cl2), whereas, with L-lactate or formate, complete dechlorination of C2Cl4 via C2HCl3, cis-C2H2Cl2 and chloroethene (C2H3Cl) to ethene was obtained. In oxic soil slurries with methane as a substrate, complete co-metabolic degradation of cis-C2H2Cl2 was obtained, whereas C2HCl3 was partially degraded. With toluene or phenol both of the above were readily co-metabolized. Complete degradation of C2Cl4 was obtained in sequentially coupled anoxic and oxic chemostats, which were inoculated with the slurry enrichments. Apparent steady states were obtained at various dilution rates (0.02-0.4 h-1) and influent C2Cl4-concentrations (100-1000 microM). In anoxic chemostats with a mixture of formate and glucose as the carbon and electron source, C2Cl4 was transformed at high rates (above 140 micromol 1-1 h-1, corresponding to 145 nmol Cl- min-1 mg protein-1), into cis-C2H2Cl2 and C2H3Cl. Reductive dechlorination was not affected by addition of 5 mM sulphate, but strongly inhibited after a...Continue Reading

Citations

Jun 5, 2004·Applied Microbiology and Biotechnology·Qiang He, Robert A Sanford
Sep 21, 2005·Applied Microbiology and Biotechnology·Norihiko TsukagoshiRyuichiro Kurane
Aug 7, 2003·Applied and Environmental Microbiology·Julien MaillardChristof Holliger
Mar 8, 2011·Current Opinion in Biotechnology·Andreas Tiehm, Kathrin R Schmidt
Jul 11, 2006·Journal of Environmental Quality·J E McLeanD L Sorensen
Dec 15, 2018·Microbial Biotechnology·Torsten SchubertGabriele Diekert
Aug 26, 2006·Water Environment Research : a Research Publication of the Water Environment Federation·George A Ehlers, Peter D Rose

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