Complete genome analysis and immunodetection of a member of a novel virus species belonging to the genus Ampelovirus

Archives of Virology
Varvara I MaliogkaN I Katis

Abstract

A new grapevine leafroll-associated virus isolate (GLRaV-Pr) from Greek grapevines was recently reported. This virus, along with the genetically related GLRaV-4, -5, -6 and -9, form a separate diverse lineage within the genus Ampelovirus. In this paper, the complete nucleotide sequence of GLRaV-Pr was determined, making it the first fully sequenced virus of this lineage. Its genome is 13,696 nt long and contains seven open reading frames, which potentially encode a 253-kDa polyprotein containing papain-like protease, methyltransferase, AlkB and helicase domains, a 58.2-kDa RNA-dependent RNA polymerase, a 5.2-kDa hydrophobic protein, a 58.5-kDa heat shock 70 protein homologue, a 60-kDa protein, a 30-kDa coat protein (CP) and a 23-kDa protein. A virus-specific antibody was raised against the recombinant CP of GLRaV-Pr and was applied in western blot analysis. The genomic, serological and phylogenetic data reported here confirm that GLRaV-Pr is a member of a distinct Ampelovirus species. Comparisons of GLRaV-Pr with the only available genetically related, fully sequenced virus, PMWaV-1, PBNSPaV and the partially sequenced GLRaV-9 revealed that this lineage, including GLRaV-4, -5, -6, -9 and -De, exhibits a high uniformity of genom...Continue Reading

References

Sep 1, 1991·The Journal of General Virology·E V Koonin
Mar 28, 1995·Proceedings of the National Academy of Sciences of the United States of America·A A AgranovskyJ G Atabekov
Jan 1, 1993·Critical Reviews in Biochemistry and Molecular Biology·E V Koonin, V V Dolja
Aug 29, 2001·Bioinformatics·J P Huelsenbeck, F Ronquist
Nov 10, 2001·Annual Review of Phytopathology·Alexander V Karasev
Oct 12, 2002·Archives of Virology·G P MartelliUNKNOWN ICTV Study Group on closteroviruses and allied viruses
Jan 10, 2003·Nucleic Acids Research·Aron Marchler-BauerStephen H Bryant
Oct 8, 2003·Systematic Biology·Stéphane Guindon, Olivier Gascuel
Jan 14, 2004·Proceedings of the National Academy of Sciences of the United States of America·Tatineni SatyanarayanaWilliam O Dawson
Mar 27, 2004·Proceedings of the National Academy of Sciences of the United States of America·Valera V PeremyslovValerian V Dolja
Jan 14, 2005·Bioinformatics·Federico AbascalDavid Posada
Feb 3, 2005·Genome Research·Chuong B DoSerafim Batzoglou
Mar 15, 2006·Virus Research·Valerian V DoljaJari P T Valkonen
Mar 17, 2007·Annual Review of Phytopathology·Giovanni P MartelliValerian V Dolja
Oct 24, 2008·Phytopathology·Anne HalgrenRobert R Martin
Mar 1, 2003·Molecular Plant Pathology·Valerian V Dolja

❮ Previous
Next ❯

Citations

Aug 13, 2010·Archives of Virology·N Abou Ghanem-SabanadzovicA Rowhani
Aug 13, 2011·Phytopathology·Olufemi J AlabiRayapati A Naidu
Nov 24, 2011·Archives of Virology·Wilhelm JelkmannGiovanni P Martelli
Apr 12, 2016·Frontiers in Plant Science·Grace ArmijoPatricio Arce-Johnson
Sep 7, 2011·Virus Research·Jeremy R ThompsonKeith L Perry

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.