Complete genome of Enterobacter sichuanensis strain SGAir0282 isolated from air in Singapore

Gut Pathogens
Akira UchidaStephan C Schuster

Abstract

Enterobacter cloacae complex (ECC) bacteria, such as E. cloacae, E. sichuanensis, E. kobei, and E. roggenkampii, have been emerging as nosocomial pathogens. Many strains isolated from medical clinics were found to be resistant to antibiotics, and in the worst cases, acquired multidrug resistance. We present the whole genome sequence of SGAir0282, isolated from the outdoor air in Singapore, and its relevance to other ECC bacteria by in silico genomic analysis. Complete genome assembly of E. sichuanensis strain SGAir0282 was generated using PacBio RSII and Illumina MiSeq platforms, and the datasets were used for de novo assembly using Hierarchical Genome Assembly Process (HGAP) and error corrected with Pilon. The genome assembly consisted of a single contig of 4.71 Mb and with a G+C content of 55.5%. No plasmid was detected in the assembly. The genome contained 4371 coding genes, 83 tRNA and 25 rRNA genes, as predicted by NCBI's Prokaryotic Genome Annotation Pipeline (PGAP). Among the genes, the antibiotic resistance related genes were included: Streptothricin acetdyltransferase (SatA), fosfomycin resistance protein (FosA) and metal-dependent hydrolases of the beta-lactamase superfamily I (BLI). Based on whole genome alignment an...Continue Reading

References

Sep 20, 2005·Diagnostic Microbiology and Infectious Disease·Harald HoffmannAndreas Roggenkamp
Aug 10, 2011·BMC Genomics·Nabil-Fareed AlikhanScott A Beatson
May 7, 2013·Nature Methods·Chen-Shan ChinJonas Korlach
Feb 2, 2015·Applied Biochemistry and Biotechnology·Jayanthi Abraham, Sivagnanam Silambarasan
Jun 5, 2015·Frontiers in Microbiology·Anne Davin-Regli, Jean-Marie Pagès
Sep 12, 2015·The ISME Journal·Frédéric RaymondJacques Corbeil
Feb 28, 2016·Molecular Biology and Evolution·Jaime Huerta-CepasPeer Bork
Jun 22, 2016·Genome Biology·Brian D OndovAdam M Phillippy
Jun 28, 2016·Nucleic Acids Research·Tatiana TatusovaJames Ostell
Jan 14, 2017·Journal of Global Antimicrobial Resistance·Bo ZhuMuhammad Ibrahim
Jun 27, 2017·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Ziad DaoudGhassan M Matar
Feb 28, 2018·Antimicrobial Agents and Chemotherapy·Ryota GomiYasufumi Matsumura
Nov 6, 2018·International Journal of Systematic and Evolutionary Microbiology·Wenjing WuZhiyong Zong
Jun 11, 2019·Microbiological Research·Fernanda Marcondes de AndradeJoyce Dória
Oct 30, 2019·Proceedings of the National Academy of Sciences of the United States of America·Elena S GusarevaStephan C Schuster

❮ Previous
Next ❯

Methods Mentioned

BETA
nucleotide collection

Software Mentioned

PacBio SMRT Analysis
Circulator
PubMLST
BLASTn
custom PERL script
kSNP3
kChooser
Gingr
Parsnp
RASTtk )

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Mitochondrial DNA. Part A. DNA Mapping, Sequencing, and Analysis
Fei ZhuPeng Guo
Ji sheng chong xue yu ji sheng chong bing za zhi = Journal of parasitology & parasitic diseases
Q D ChenF R Zeng
Sichuan yi xue yuan xue bao = Acta Academiae Medicinae Sichuan
Y F PengP N Liu
© 2021 Meta ULC. All rights reserved