Complete Genome of the Xanthomonas euvesicatoria Specific Bacteriophage KΦ1, Its Survival and Potential in Control of Pepper Bacterial Spot

Frontiers in Microbiology
Katarina GašićAleksa Obradović

Abstract

Xanthomonas euvesicatoria phage KΦ1, a member of Myoviridae family, was isolated from the rhizosphere of pepper plants showing symptoms of bacterial spot. The phage strain expressed antibacterial activity to all X. euvesicatoria strains tested and did not lyse other Xanthomonas spp., nor other less related bacterial species. The genome of KΦ1 is double-stranded DNA of 46.077 bp including 66 open reading frames and an average GC content of 62.9%, representing the first complete genome sequence published for a phage infecting xanthomonads associated with pepper or tomato. The highest genome similarity was observed between phage KΦ1 and the Xanthomonas oryzae pv. oryzae specific phage OP2. On the other hand, when compared with other members of the genus Bcep78virus, the genome similarity was lower. Forty-four (67%) predicted KΦ1 proteins shared homology with Xanthomonas phage OP2, while 20 genes (30%) were unique to KΦ1. Phage KΦ1, which is chloroform resistant and stable in different media and in the pH range 5-11, showed a high titer storage ability for at least 2 years at +4°C. Copper-hydroxide and copper-oxychloride reduced phage activity proportionally to the used concentrations and the exposure time. UV light was detrimental...Continue Reading

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Citations

May 10, 2020·Microbial Biotechnology·Mojgan RabieyRobert W Jackson
Sep 30, 2020·Microorganisms·Ivana PajčinJovana Grahovac
Nov 12, 2020·Current Opinion in Biotechnology·Dominique HoltappelsJeroen Wagemans
Sep 3, 2021·Molecular Plant Pathology·Ebrahim OsdaghiGary E Vallad

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Datasets Mentioned

BETA
KY210139

Methods Mentioned

BETA
electrophoresis

Software Mentioned

BLASTP
CoreGenes
ARAGORN
Mauve
IGS Annotation Engine
SE
RAST ( Rapid Annotation using Technology
tRNAscan
SnapGene
VirulentPred

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