Complete genome sequence analysis of dengue virus type 2 isolated in Brunei

Virus Research
Osmali OsmanShamala Devi

Abstract

In a previous study, we have reported the detection and isolation of dengue virus in Brunei (Osman, O., Fong, M.Y., Devi, S., 2007. A preliminary study of dengue infection in Brunei. JJID 60 (4), 205-208). DEN-2 was the predominant serotype followed by DEN-1. The full genomic sequences of 3 DEN-2 viruses isolated during the 2005-2006 dengue incident in Brunei were determined. Twenty-five primer sets were designed to amplify contiguous overlapping fragments of approximately 500-600 base pairs spanning the entire sequence of the viral genome. The amplified PCR products were sent for sequencing and their nucleotides and the deduced amino acids were determined. All three DEN-2 virus isolated were clustered in the Cosmopolitan genotype of the DEN-2 classification by Twiddy et al. This work constitutes the first complete genetic characterization of three Brunei DEN-2 virus strains.

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Citations

Nov 26, 2010·Molecular Biology Reports·Muhammad FaheemIshtiaq Qadri
Oct 17, 2013·Journal of Virological Methods·Patcharawan SujayanontPrapat Suriyaphol
Jan 15, 2014·Asian Pacific Journal of Tropical Medicine·Joy Ann G PetronioRaul V Destura
Sep 30, 2018·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Shivani SukhraliaChhaya Ravi Kant

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