Complete genome sequence and characterization of avian pathogenic Escherichia coli field isolate ACN001

Standards in Genomic Sciences
Xiangru WangChen Tan

Abstract

Avian pathogenic Escherichia coli is an important etiological agent of avian colibacillosis, which manifests as respiratory, hematogenous, meningitic, and enteric infections in poultry. It is also a potential zoonotic threat to human health. The diverse genomes of APEC strains largely hinder disease prevention and control measures. In the current study, pyrosequencing was used to analyze and characterize APEC strain ACN001 (= CCTCC 2015182(T) = DSMZ 29979(T)), which was isolated from the liver of a diseased chicken in China in 2010. Strain ACN001 belongs to extraintestinal pathogenic E. coli phylogenetic group B1, and was highly virulent in chicken and mouse models. Whole genome analysis showed that it consists of six different plasmids along with a circular chromosome of 4,936,576 bp, comprising 4,794 protein-coding genes, 108 RNA genes, and 51 pseudogenes, with an average G + C content of 50.56 %. As well as 237 coding sequences, we identified 39 insertion sequences, 12 predicated genomic islands, 8 prophage-related sequences, and 2 clustered regularly interspaced short palindromic repeats regions on the chromosome, suggesting the possible occurrence of horizontal gene transfer in this strain. In addition, most of the virulen...Continue Reading

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Datasets Mentioned

BETA
UMNK88
CP007442
PRJNA234088
KC853434
KC853435
KC853436
KC853437
KC853438

Methods Mentioned

BETA
electrophoresis
PCR

Software Mentioned

Consed
tRNAscan
SE
Newbler
MEGA
SignalP
Cytoscape
GeneMark
CR
Phred

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