Complete genome sequence of Borrelia afzelii K78 and comparative genome analysis

PloS One
Wolfgang SchülerUrban Lundberg

Abstract

The main Borrelia species causing Lyme borreliosis in Europe and Asia are Borrelia afzelii, B. garinii, B. burgdorferi and B. bavariensis. This is in contrast to the United States, where infections are exclusively caused by B. burgdorferi. Until to date the genome sequences of four B. afzelii strains, of which only two include the numerous plasmids, are available. In order to further assess the genetic diversity of B. afzelii, the most common species in Europe, responsible for the large variety of clinical manifestations of Lyme borreliosis, we have determined the full genome sequence of the B. afzelii strain K78, a clinical isolate from Austria. The K78 genome contains a linear chromosome (905,949 bp) and 13 plasmids (8 linear and 5 circular) together presenting 1,309 open reading frames of which 496 are located on plasmids. With the exception of lp28-8, all linear replicons in their full length including their telomeres have been sequenced. The comparison with the genomes of the four other B. afzelii strains, ACA-1, PKo, HLJ01 and Tom3107, as well as the one of B. burgdorferi strain B31, confirmed a high degree of conservation within the linear chromosome of B. afzelii, whereas plasmid encoded genes showed a much larger diver...Continue Reading

References

Jan 1, 1988·Annals of the New York Academy of Sciences·A G Barbour, C F Garon
Mar 1, 1988·Journal of Clinical Microbiology·A G Barbour
Nov 1, 1987·The Journal of Infectious Diseases·T G Schwan, W Burgdorfer
Jun 1, 1984·Die Naturwissenschaften·H H AlthausP Schwartz
Sep 1, 1995·Infection and Immunity·B P GuoM Höök
Aug 1, 1996·The Journal of Infectious Diseases·A OhlenbuschH Eiffert
Jun 1, 1996·Molecular Biotechnology·R Staden
Dec 24, 1998·Nucleic Acids Research·G Benson
Mar 27, 1999·Molecular Microbiology·B P GuoM Höök
Nov 11, 1999·Nucleic Acids Research·A L DelcherS L Salzberg
Nov 26, 1999·Journal of Bacteriology·C H Eggers, D S Samuels
Dec 11, 1999·Nucleic Acids Research·R L TatusovE V Koonin
May 29, 2000·Trends in Genetics : TIG·P RiceA Bleasby
Dec 6, 2000·Proceedings of the National Academy of Sciences of the United States of America·J E Purser, S J Norris
Dec 20, 2000·Bioinformatics·K RutherfordB Barrell
Jul 14, 2001·The New England Journal of Medicine·A C Steere
Jul 24, 2001·Journal of Bacteriology·C H EggersD S Samuels
Mar 28, 2002·Nucleic Acids Research·A J EnrightC A Ouzounis
Jan 10, 2003·Nucleic Acids Research·Daniel H HaftOwen White

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Citations

Mar 28, 2018·BMC Genomics·Sherwood R CasjensWei-Gang Qiu
Jan 7, 2018·Applied and Environmental Microbiology·Luis M Rodriguez-RKonstantinos T Konstantinidis
Jun 12, 2018·PloS One·Neda JabbariChristopher G Lausted
Jun 27, 2018·PloS One·Sébastien Bontemps-GalloFrank C Gherardini

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Datasets Mentioned

BETA
CP000395
AF008219

Methods Mentioned

BETA
biopsy
lipidation
PCR

Software Mentioned

PSORTb
TriTISA
HAPS
Tandem Repeat Finder
Jalview
Staden
InterProScan
rpsblast
CGview
ClustalW

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