Abstract
Chinese strong-flavor liquor (CSFL), accounting for more than 70% of both Chinese liquor production and sales, was produced by complex fermentation with pit mud. Clostridium kluyveri, an important species coexisted with other microorganisms in fermentation pit mud (FPM), could produce caproic acid, which was subsequently converted to the key CSFL flavor substance ethyl caproate. In this study, we present the first complete genome sequence of C. kluyveri isolated from FPM. Clostridium kluyveri JZZ contains one circular chromosome and one circular plasmid with length of 4,454,353 and 58,581 bp, respectively. 4158 protein-coding genes were predicted and 2792 genes could be assigned with COG categories. It possesses the pathway predicted for biosynthesis of caproic acid with ethanol. Compared to other two C. kluyveri genomes, JZZ consists of longer chromosome with multiple gene rearrangements, and contains more genes involved in defense mechanisms, as well as DNA replication, recombination, and repair. Meanwhile, JZZ contains fewer genes involved in secondary metabolites biosynthesis, transport, and catabolism, including genes encoding Polyketide Synthases/Non-ribosomal Peptide Synthetases. Additionally, JZZ possesses 960 unique ge...Continue Reading
References
Jul 3, 2004·Genome Research·Aaron C E DarlingNicole T Perna
Jun 28, 2005·Nucleic Acids Research·Peter SchattnerTodd M Lowe
Mar 1, 1942·Journal of Bacteriology·H A Barker, S M Taha
Apr 25, 2007·Nucleic Acids Research·Karin LagesenDavid W Ussery
Jan 26, 2008·Proceedings of the National Academy of Sciences of the United States of America·Henning SeedorfGerhard Gottschalk
Apr 16, 2008·Nucleic Acids Research·Jason R Grant, Paul Stothard
Jun 10, 2009·Bioinformatics·Heng LiUNKNOWN 1000 Genome Project Data Processing Subgroup
Oct 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Michael Richter, Ramon Rosselló-Móra
Jul 2, 2010·PloS One·Aaron E DarlingNicole T Perna
Dec 2, 2011·Bioinformatics·Yongbing ZhaoJun Yu
Dec 14, 2011·Applied Microbiology and Biotechnology·Paul J Weimer, David M Stevenson
Mar 6, 2012·Nature Methods·Ben Langmead, Steven L Salzberg
May 7, 2013·Nature Methods·Chen-Shan ChinJonas Korlach
Feb 4, 2014·Applied and Environmental Microbiology·Yong TaoXiangzhen Li
Apr 29, 2014·Journal of Biotechnology·Yuna CheonDae-Hyuk Kweon
Nov 22, 2014·Nucleic Acids Research·Aron Marchler-BauerStephen H Bryant
Dec 3, 2014·Bioresource Technology·Paul J WeimerDane J Brandl
May 8, 2015·Nucleic Acids Research·Tilmann WeberMarnix H Medema
Aug 13, 2015·International Journal of Food Microbiology·Xiao-Long HuYan Xu
Oct 23, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jihong WuMingquan Huang
Feb 21, 2016·Applied and Environmental Microbiology·Xiaolong HuYan Xu
Jun 28, 2016·Nucleic Acids Research·Tatiana TatusovaJames Ostell
Nov 24, 2016·Frontiers in Microbiology·Hanno RichterLargus T Angenent
Feb 22, 2017·Journal of Biotechnology·Gonzalo Torres TejerizoAndreas Schlüter
Apr 4, 2017·Biotechnology for Biofuels·Sylvia GildemynLargus T Angenent