Complete genome sequence of longan witches' broom-associated virus, a novel member of the family Potyviridae

Archives of Virology
Jang-Kyun SeoHong Soo Choi

Abstract

The complete genome sequence of a new virus isolated from a longan (Dimocarpus longan Lour.) plant showing witches' broom syndrome was determined. The viral genome is composed of a monopartite single-stranded RNA of 9,428 nucleotides excluding the 3' poly(A) tail and contains one large single open reading frame encoding a polyprotein of 3086 amino acids. BLAST searches of protein databases showed that the encoded polyprotein has a maximum amino acid sequence identity of 35% (with 85% coverage) to that of the isolate Minnesota of rose yellow mosaic virus (RoYMV; family Potyviridae; genus not assigned). Molecular and phylogenetic analysis of the genome and encoded protein sequences showed that the identified virus has the general features that are characteristic of members of the family Potyviridae although it has extremely low sequence similarity to known members of the family Potyviridae. The name longan witches' broom-associated virus (LWBaV) is proposed for this new virus, which may be considered a member of a new genus in the family Potyviridae.

References

Oct 16, 2007·Virus Research·James SusaimuthuRobert R Martin
Oct 8, 2013·PLoS Computational Biology·Sven-Eric SchelhornFrank Berthold
Mar 24, 2016·Molecular Biology and Evolution·Sudhir KumarKoichiro Tamura

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Datasets Mentioned

BETA
KY649478

Methods Mentioned

BETA
PCR

Software Mentioned

BLASTx
BLASTn
MEGA7
ClustalX
Trinity

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