Complete genome sequence of the potato pathogen Ralstonia solanacearum UY031

Standards in Genomic Sciences
Rodrigo Guarischi-SousaJoão C Setubal

Abstract

Ralstonia solanacearum is the causative agent of bacterial wilt of potato. Ralstonia solanacearum strain UY031 belongs to the American phylotype IIB, sequevar 1, also classified as race 3 biovar 2. Here we report the completely sequenced genome of this strain, the first complete genome for phylotype IIB, sequevar 1, and the fourth for the R. solanacearum species complex. In addition to standard genome annotation, we have carried out a curated annotation of type III effector genes, an important pathogenicity-related class of genes for this organism. We identified 60 effector genes, and observed that this effector repertoire is distinct when compared to those from other phylotype IIB strains. Eleven of the effectors appear to be nonfunctional due to disruptive mutations. We also report a methylome analysis of this genome, the first for a R. solanacearum strain. This analysis helped us note the presence of a toxin gene within a region of probable phage origin, raising the hypothesis that this gene may play a role in this strain's virulence.

References

Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·C R WoeseM L Wheelis
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
Sep 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·F García-Del PortilloJ Casadesús
Feb 2, 2002·Nature·M SalanoubatC A Boucher
Sep 4, 2003·Genome Research·Li LiDavid S Roos
Mar 23, 2004·Nucleic Acids Research·Robert C Edgar
Jan 13, 2006·International Journal of Systematic and Evolutionary Microbiology
Jan 13, 2006·Molecular Plant-microbe Interactions : MPMI·Dean W GabrielNatalia Mikhailova
May 10, 2008·Nature Biotechnology·Dawn FieldAnil Wipat
May 11, 2010·Nature Methods·Benjamin A FlusbergStephen W Turner
Nov 9, 2010·Journal of Applied Microbiology·S MurugaiyanS-W Lee
May 9, 2012·Annual Review of Phytopathology·Stéphane Genin, Timothy P Denny
Jun 8, 2012·Molecular Plant Pathology·John MansfieldGary D Foster
May 7, 2013·Nature Methods·Chen-Shan ChinJonas Korlach
Oct 8, 2013·Applied and Environmental Microbiology·Bruno Contreras-Moreira, Pablo Vinuesa
Mar 20, 2014·Bioinformatics·Torsten Seemann
Nov 8, 2014·Nucleic Acids Research·Richard J RobertsDana Macelis
Mar 31, 2015·Current Opinion in Microbiology·María A Sánchez-RomeroJosep Casadesús

❮ Previous
Next ❯

Citations

Sep 27, 2018·Molecular Plant Pathology·André da Silva XavierPoliane Alfenas-Zerbini
Apr 5, 2017·Frontiers in Plant Science·Marina PuigvertMarc Valls
Apr 30, 2017·Frontiers in Plant Science·Ivan ErillMarc Valls

❮ Previous
Next ❯

Datasets Mentioned

BETA
SRZ132405
CP012687
CP012688
PRJNA278086
GM1000

Methods Mentioned

BETA
light microscopy

Software Mentioned

RS
MUSCLE
_ Modification Motif
orthoMCL
BLAST
SMRT Analysis
Resequencing
Ralstonia T3E
MIGS
PhyML

Related Concepts

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.