Complete genome sequences of hepatitis C virus subtype 3i and 3a subtype isolates from India.

Indian Journal of Gastroenterology : Official Journal of the Indian Society of Gastroenterology
Gouri M GupteVidya A Arankalle

Abstract

Hepatitis C virus (HCV), a major causative agent of chronic hepatitis, is classified into six major genotypes. Genotype 3 HCV infection is more sensitive to interferon therapy. In India, genotype 3, particularly subtype 3a, HCV infections are common. Three novel HCV subtypes i.e., 3g, 3j, and 3i were identified from India based on partial genomic sequences. This report provides full genome sequences of one isolate each of subtypes 3i and 3a. Serum samples positive for subtype 3i and 3a HCV RNA based on core region genomic sequences were studied. Complete HCV genomes were amplified as 11 overlapping PCR fragments and sequenced. The complete genomic sequence of Indian HCV 3i isolate clustered with other genotype 3 sequences, and was closer to subtypes 3b and 3a (80.5% and 79.1% [SD 0.4%] nucleotide identity). Nucleotide similarities were the highest in the core region (86.1-88.7%), and the least in the E2 region (69.4-70.7%). Phylogenetic tree analysis confirmed the existence of a separate subtype 3i. The Indian HCV 3a isolate's complete genomic sequences clustered with previously known genotype 3a sequences with a nucleotide similarity of 91.1% (SD 0.2%). Neither isolates showed evidence of recombination of different HCV genotyp...Continue Reading

References

Jan 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J BukhR H Miller
Jan 1, 1974·Clinical Toxicology
Mar 1, 1995·The Journal of General Virology·T ValliammaiT J Harrison
Feb 1, 1996·Journal of Medical Virology·A K PanigrahiS K Panda
Apr 12, 2000·Journal of Viral Hepatitis·D B Smith
Jul 21, 2004·Briefings in Bioinformatics·Sudhir KumarMasatoshi Nei
Sep 21, 2004·Bioinformatics·Carla KuikenRussell Richardson
Oct 16, 2004·The Journal of General Virology·Peter Simmonds
Sep 9, 2005·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Peter SimmondsAnders Widell
Nov 29, 2005·Applied Bioinformatics·Karina YusimCarla Kuiken
May 23, 2009·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Shobha NarahariDhananjaya Saranath

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Citations

Nov 10, 2011·Viruses·Fernando González-CandelasMaría Alma Bracho
Apr 23, 2013·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Gianguglielmo ZehenderMassimo Ciccozzi

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