Complete genome sequencing and analysis of endophytic Sphingomonas sp. LK11 and its potential in plant growth

3 Biotech
Sajjad AsafIn-Jung Lee

Abstract

Our study aimed to elucidate the plant growth-promoting characteristics and the structure and composition of Sphingomonas sp. LK11 genome using the single molecule real-time (SMRT) sequencing technology of Pacific Biosciences. The results revealed that LK11 produces different types of gibberellins (GAs) in pure culture and significantly improves soybean plant growth by influencing endogenous GAs compared with non-inoculated control plants. Detailed genomic analyses revealed that the Sphingomonas sp. LK11 genome consists of a circular chromosome (3.78 Mbp; 66.2% G+C content) and two circular plasmids (122,975 bps and 34,160 bps; 63 and 65% G+C content, respectively). Annotation showed that the LK11 genome consists of 3656 protein-coding genes, 59 tRNAs, and 4 complete rRNA operons. Functional analyses predicted that LK11 encodes genes for phosphate solubilization and nitrate/nitrite ammonification, which are beneficial for promoting plant growth. Genes for production of catalases, superoxide dismutase, and peroxidases that confer resistance to oxidative stress in plants were also identified in LK11. Moreover, genes for trehalose and glycine betaine biosynthesis were also found in LK11 genome. Similarly, Sphingomonas spp. analysi...Continue Reading

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Citations

Jan 16, 2019·Plant, Cell & Environment·Yongjia ZhongHong Liao
Aug 10, 2019·Microbiology Resource Announcements·Rachel RathsHeike Bücking
Jan 31, 2020·World Journal of Microbiology & Biotechnology·Prity KushwahaRajesh Kumar Tiwari
Jun 20, 2020·Microbiology Resource Announcements·Swati BijlaniKasthuri Venkateswaran
Jan 28, 2021·International Journal of Environmental Research and Public Health·Mampolelo M PhotoloMatsobane G Tlou
Jan 29, 2021·Microbiological Research·Sravanthi Goud Burragoni, Junhyun Jeon
Apr 10, 2021·Journal of Basic Microbiology·Surjit S DudejaErika Kothe
Jul 31, 2021·Journal of Hazardous Materials·Pankaj BhattShaohua Chen

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Datasets Mentioned

BETA
ICMP
21288

Software Mentioned

GView
SMRT Portal
BLAST +
EDGAR
QUAST
Ring Image Generator ( BRIG
SMRT Analysis
Interactive Microbial Genome Visualization with
Rapid Annotation using Subsystem Technology ( RAST )
PacBio

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