Complete genome sequencing of Pandoraea pnomenusa RB38 and Molecular Characterization of Its N-acyl homoserine lactone synthase gene ppnI

PeerJ
Yan-Lue LimKok-Gan Chan

Abstract

In this study, we sequenced the genome of Pandoraea pnomenusa RB38 using Pacific Biosciences RSII (PacBio) Single Molecule Real Time (SMRT) sequencing technology. A pair of cognate luxI/R homologs was identified where the luxI homolog, ppnI, was found adjacent to a luxR homolog, ppnR1. An additional orphan luxR homolog, ppnR2, was also discovered. Multiple sequence alignment and phylogenetic analysis revealed that ppnI is an N-acyl homoserine lactone (AHL) synthase gene that is distinct from those of the nearest phylogenetic neighbor viz. Burkholderia spp. High resolution tandem mass spectrometry (LC-MS/MS) analysis showed that Escherichia coli BL21 harboring ppnI produced a similar AHL profile (N-octanoylhomoserine lactone, C8-HSL) as P. pnomenusa RB38, the wild-type donor strain, confirming that PpnI directed the synthesis of AHL in P. pnomenusa RB38. To our knowledge, this is the first documentation of the luxI/R homologs of the genus Pandoraea.

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Citations

Jan 29, 2016·Lab on a Chip·Sarah M FriedrichTza-Huei Wang
May 25, 2016·Expert Review of Anti-infective Therapy·Kok-Gan Chan
Mar 29, 2020·Microbiology Resource Announcements·Miranda E PittLachlan J M Coin

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Datasets Mentioned

BETA
CP007506.1
AHN77102.1
AHN77101.1

Methods Mentioned

BETA
biosensors
fluorescence microscopy
biosensor

Software Mentioned

FIGfam
BLAST
ClustalW
MapSolver
EzTaxon e - analysis
MEGA5
RAST
Gepard
MEGA6
Enzyme Chooser

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