Complete Genomic Analysis of a Salmonella enterica Serovar Typhimurium Isolate Cultured From Ready-to-Eat Pork in China Carrying One Large Plasmid Containing mcr-1

Frontiers in Microbiology
Wei WangSéamus Fanning

Abstract

One mcr-1-carrying ST34-type Salmonella Typhimurium WW012 was cultured from 3,200 ready-to-eat (RTE) pork samples in 2014 in China. Broth dilution method was applied to obtain the antimicrobial susceptibility of Salmonella Typhimurium WW012. Broth matting assays were carried out to detect transferability of this phenotype and whole-genome sequencing was performed to analyze its genomic characteristic. Thirty out of 3,200 RTE samples were positive for Salmonella and the three most frequent serotypes were identified as S. Derby (n = 8), S. Typhimurium (n = 6), and S. Enteritidis (n = 6). One S. Typhimurium isolate (S. Typhimurium WW012) cultured from RTE prepared pork was found to contain the mcr-1 gene. S. Typhimurium WW012 expressed a level of high resistance to seven different antimicrobial compounds in addition to colistin (MIC = 8 mg/L). A single plasmid, pWW012 (151,609-bp) was identified and found to be of an IncHI2/HI2A type that encoded a mcr-1 gene along with six additional antimicrobial resistance genes. Plasmid pWW012 contained an IS30-mcr-1-orf-orf-IS30 composite transposon that can be successfully transferred to Escherichia coli J53. When assessed further, the latter demonstrated considerable similarity to three pla...Continue Reading

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Citations

Mar 3, 2020·Environmental Microbiology·Yingbo ShenYang Wang
Jan 3, 2019·The Journal of Antimicrobial Chemotherapy·Yujie HuFengqin Li
Apr 16, 2020·Advanced Biosystems·Biao TangYoujun Feng
Sep 5, 2021·Journal of Applied Microbiology·Ana Beatriz PortesPedro Panzenhagen

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Datasets Mentioned

BETA
AE006468.1
CP015724
CP022168
CP022169

Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

BLASTP
PlasmidFinder
BLASTn
RAST

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