PMID: 11328646May 1, 2001Paper

Complete genomic sequence of 195 Kb of human DNA containing the gene GABRG2

DNA Sequence : the Journal of DNA Sequencing and Mapping
S JiangH Yang

Abstract

GABA (gamma-aminobutyric acid), as the main inhibitory neurotransmitter in the brain, plays an essential role for the overall balance between neuronal excitation and inhibition by acting on GABAA receptors, which are ligand-gated chloride channels. Impaired GABAergic function contributes to certain forms of epilepsy, schizophrenia, Alzheimer's Disease, and other neurological disorders. In order to identify possible genetic features and to further study biological regulation of GABAA receptor genes whose promoter elements and sequence anomalies may contribute to epileptic disorders, as an initial step, we shot-gun sequenced a BAC clone, dj082c10 (195,909-bp in size), encompassing human gamma(2) subunit of GABAA receptor (GABRG2). It is, we believe, the first genomic sequence of the GABA receptor gamma subunit family. Four contigs were assembled from 2950 reads prior to gap in an average redundancy of eight folds over the entire region. The precision of the consensus sequence was predicted to be 99.999% after closing gaps and finishing weak regions. The nine exons of GABRG2 spans an 85-kb region that had 81 SINEs comprising 22.32%, and nine L1 elements comprising 3.40%, respectively. However, the density of L1 in the regions flan...Continue Reading

References

May 1, 1992·European Journal of Epidemiology·M R McGinnisH L Lucia
Mar 1, 1991·Neurochemical Research·J G Tasker, F E Dudek
Oct 4, 1991·Science·T HunkapillerL Hood
Feb 27, 1989·FEBS Letters·P R SchofieldP H Seeburg
Jan 1, 1994·Annual Review of Neuroscience·R L Macdonald, R W Olsen
Mar 1, 1995·Neuropharmacology·H LüddensP H Seeburg
Jun 23, 1995·Journal of Molecular Biology·D S Prestridge
Jan 1, 1996·European Journal of Human Genetics : EJHG·M KostrzewaU Müller
May 13, 1997·Proceedings of the National Academy of Sciences of the United States of America·G K WongM V Olson
Jan 1, 1995·Journal of Peptide Science : an Official Publication of the European Peptide Society·C TonioloG L Millhauser
May 16, 1998·Genome Research·D GordonP Green
May 8, 1999·Gene·J E Tabaska, M Q Zhang

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Citations

Nov 19, 2003·Journal of Clinical Psychopharmacology·Adel WassefLisa D Kochan

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