Complete human gene structure of obscurin: implications for isoform generation by differential splicing.

Journal of Muscle Research and Cell Motility
Atsushi FukuzawaMathias Gautel

Abstract

The complete gene giant muscle protein obscurin, a modular protein composed largely of tandem Ig-domains, GDP/GTP exchange factor domains (GEF) for small G-proteins, and differentially spliced kinase domains, was analysed. The splice donor and acceptor sites of the 117 exons give important clues for potential splice pathways. The fusion of the conventional obscurin A, containing only the GEF domain, and obscurin B, fusing into the 3' kinase exons, was experimentally confirmed and analysed. The linker between the two kinases contains multiple predicted phosphorylation sites, as well as a predicted NFX zinc finger domain. Both kinases show only weak homology to either myosin light chain kinases or other giant muscle protein kinases, suggesting that they are functionally distinct.

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Citations

Mar 19, 2011·Pflügers Archiv : European journal of physiology·Mathias Gautel
Sep 4, 2008·Proceedings of the National Academy of Sciences of the United States of America·Elias M PuchnerMathias Gautel
Sep 11, 2008·The Journal of Biological Chemistry·Shane R Cunha, Peter J Mohler
May 1, 2009·Molecular Biology of the Cell·Maegen A AckermannAikaterini Kontrogianni-Konstantopoulos
Oct 1, 2009·Physiological Reviews·Aikaterini Kontrogianni-KonstantopoulosRobert J Bloch
Apr 24, 2010·Journal of Biomedicine & Biotechnology·Hiroshi Qadota, Guy M Benian
Jul 9, 2009·Journal of Cell Science·Stephan LangeJu Chen
Feb 12, 2014·PloS One·Maegen A AckermannAikaterini Kontrogianni-Konstantopoulos
Feb 9, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Li-Yen R Hu, Aikaterini Kontrogianni-Konstantopoulos
Feb 20, 2013·The Journal of Cell Biology·Davide RandazzoVincenzo Sorrentino
May 17, 2017·Biophysics Reviews·Heather R ManringMaegen A Ackermann
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Mar 27, 2007·FEBS Letters·Amber L BowmanRobert J Bloch
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