Complete in vitro assembly of the reovirus outer capsid produces highly infectious particles suitable for genetic studies of the receptor-binding protein

Journal of Virology
Kartik ChandranMax L Nibert

Abstract

Mammalian reoviruses, prototype members of the Reoviridae family of nonenveloped double-stranded RNA viruses, use at least three proteins--sigma1, mu1, and sigma3--to enter host cells. sigma1, a major determinant of cell tropism, mediates viral attachment to cellular receptors. Studies of sigma1 functions in reovirus entry have been restricted by the lack of methodologies to produce infectious virions containing engineered mutations in viral proteins. To mitigate this problem, we produced virion-like particles by "recoating" genome-containing core particles that lacked sigma1, mu1, and sigma3 with recombinant forms of these proteins in vitro. Image reconstructions from cryoelectron micrographs of the recoated particles revealed that they closely resembled native virions in three-dimensional structure, including features attributable to sigma1. The recoated particles bound to and infected cultured cells in a sigma1-dependent manner and were approximately 1 million times as infectious as cores and 0.5 times as infectious as native virions. Experiments with recoated particles containing recombinant sigma1 from either of two different reovirus strains confirmed that differences in cell attachment and infectivity previously observed...Continue Reading

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Citations

Aug 14, 2003·Trends in Microbiology·Kartik Chandran, Max L Nibert
Jul 26, 2008·Proceedings of the National Academy of Sciences of the United States of America·Melina A AgostoMax L Nibert
Jan 12, 2011·Proceedings of the National Academy of Sciences of the United States of America·Lingpeng ChengPing Zhu
May 1, 2013·Proceedings of the National Academy of Sciences of the United States of America·Aleksander A DemidenkoMax L Nibert
Sep 29, 2006·Journal of Virology·Lan ZhangStephen C Harrison
Sep 15, 2006·Journal of Virology·Shane D Trask, Philip R Dormitzer
Nov 7, 2012·Current Opinion in Virology·Max L Nibert, Yuko Takagi
Mar 27, 2012·Current Opinion in Virology·Emily Happy Miller, Kartik Chandran
Jul 16, 2008·Journal of Molecular Biology·Lingpeng ChengZ Hong Zhou
Aug 3, 2005·Biochemical and Biophysical Research Communications·Yuko TsurutaDavid T Curiel
Dec 29, 2009·Journal of Molecular Biology·Lingpeng ChengZ Hong Zhou
Dec 11, 2014·Annual Review of Biochemistry·Stephen C Harrison
Jan 12, 2005·Structure·Fauziah Mohd JaafarXavier De Lamballerie
Jan 16, 2007·Virus Research·Yun ZhangChunguo Liu
Aug 3, 2018·Journal of Virology·Deepti Thete, Pranav Danthi
Oct 22, 2003·Canadian Journal of Microbiology·Etienne Rouault, Guy Lemay
Nov 5, 2020·Cancers·Louise MüllerFiona Errington-Mais

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