Complete nucleotide sequence of the structural gene for alkaline proteinase from Pseudomonas aeruginosa IFO 3455.

Infection and Immunity
K OkudaJ Fukushima

Abstract

The DNA-encoding alkaline proteinase (AP) of Pseudomonas aeruginosa IFO 3455 was cloned, and its complete nucleotide sequence was determined. When the cloned gene was ligated to pUC18, the Escherichia coli expression vector, the gene-incorporated bacteria expressed high levels of both AP activity and AP antigens. The amino acid sequence deduced from the nucleotide sequence revealed that the mature AP consists of 467 amino acids with a relative molecular weight of 49,507. The amino acid composition predicted from the DNA sequence was similar to the chemically determined composition of purified AP reported previously. The amino acid sequence analysis revealed that both the N-terminal side sequence of the purified AP and several internal lysyl peptide fragments were identical to the deduced amino acid sequences. The percent homology of amino acid sequences between AP and Serratia protease was about 55%. The zinc ligands and an active site of the AP were predicted by comparing the structure of the enzyme with of Serratia protease, thermolysin, Bacillus subtilis neutral protease, and Pseudomonas elastase.

References

Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Oct 1, 1987·Journal of Bacteriology·J B Goldberg, D E Ohman
Feb 1, 1986·Journal of Bacteriology·B B FinlayW Paranchych
May 1, 1986·Infection and Immunity·M L VasilC C Grant
Jul 25, 1986·Nucleic Acids Research·K NakahamaH Matsubara
Jun 1, 1987·Journal of Bacteriology·P A SchadB H Iglewski
May 16, 1988·Biochemical and Biophysical Research Communications·S YamamotoK Morihara
Jun 11, 1986·Nucleic Acids Research·G von Heijne
Jun 6, 1973·Biochimica Et Biophysica Acta·K MoriharaT Oka
Apr 1, 1966·Archives of Biochemistry and Biophysics·K Morihara, H Tsuzuki
Jan 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·T R Gingeras, J E Brooks
Jan 1, 1982·Annual Review of Microbiology·S Michaelis, J Beckwith
May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle
Sep 1, 1964·Journal of Bacteriology·K MORIHARA
Jul 12, 1972·Nature: New Biology·K TitaniH Neurath
Jul 12, 1972·Nature: New Biology·B W MatthewsD Dupourque

❮ Previous
Next ❯

Citations

Jan 19, 2010·Journal of Basic Microbiology·Raja Noor Zaliha Raja Abd RahmanAbu Bakar Salleh
Oct 11, 2001·Microbiology and Immunology·T ShigematsuK Okuda
Oct 9, 2002·Molecular Microbiology·Artur SchmidtchenLars Björck
Jun 22, 1999·Journal of Molecular Biology·Y ZhangK Geider
Jun 1, 1995·Current Opinion in Structural Biology·W Stöcker, W Bode
Mar 22, 2016·Plasmid·Eigo TakahashiKazuhiro Iiyama
Nov 1, 1992·Research in Microbiology·J M Ghigo, C Wandersman
Dec 1, 1993·Microbiological Reviews·C C Häse, R A Finkelstein

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.