Complete nucleotide sequences of four dsRNAs associated with a new chrysovirus infecting Aspergillus fumigatus.

Virus Research
Atif JamalRobert H A Coutts

Abstract

A new double-stranded RNA (dsRNA) virus designated A. fumigatus chrysovirus (AfuCV), belonging to the family Chrysoviridae, has been identified in the filamentous fungus Aspergillus fumigatus. The virus was detected in five of 390 A. fumigatus isolates screened. Analysis of purified dsRNA revealed four distinct species 3560, 3159, 3006 and 2863 base pairs in length (dsRNAs 1-4) which were cloned and sequenced. Each dsRNA contains a single open reading frame (ORF) with short 5' and 3' untranslated regions containing strictly conserved termini. The deduced 1114 amino acid (aa) protein (molecular mass=128 kDa) encoded by the dsRNA1 ORF showed homology to the RNA-dependent RNA polymerase (RdRP) of viruses belonging to the Chrysoviridae. Eight motifs characteristic of RdRPs were identified. The dsRNA2 ORF encodes the putative coat protein subunit (953aa; molecular mass=107 kDa). The dsRNA3 and dsRNA4 ORFs respectively encode putative proteins (891aa, molecular mass=99 kDa) and (847aa, molecular mass=95 kDa), both of which have significant similarity to proteins encoded by comparable chrysovirus dsRNAs. The dsRNA profile, amino acid sequence alignments, and phylogenetic analyses all indicate that AfuCV is a new species within the fam...Continue Reading

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Citations

Apr 15, 2011·Uirusu·Sotaro ChibaNobuhiro Suzuki
Dec 1, 2011·Indian Journal of Virology : an Official Organ of Indian Virological Society·Sakshi SharmaJ N Shrivastava
Jun 15, 2011·Archives of Virology·Muhammad Faraz BhattiRobert H A Coutts
Jul 5, 2012·Archives of Virology·Zisis KozlakidisRobert H A Coutts
Jan 28, 2015·Fungal Genetics and Biology : FG & B·Selin Özkan, Robert H A Coutts
Jul 4, 2015·Proceedings of the National Academy of Sciences of the United States of America·Lakkhana KanhayuwaRobert H A Coutts
Aug 16, 2011·Fungal Genetics and Biology : FG & B·Muhammad F BhattiRobert H A Coutts
Jul 9, 2011·Virus Research·Noemí Herrero, Iñigo Zabalgogeazcoa
Jan 24, 2015·Archives of Virology·Unnati A ShahRobert H A Coutts
May 9, 2018·Viruses·Selin Özkan-Kotiloğlu, Robert H A Coutts
Oct 12, 2018·Journal of Virology·Mahtab PeyambariMarilyn J Roossinck
Mar 1, 2019·Medical Mycology·Wendy W J van de Sande, Alieke G Vonk
May 23, 2012·Mycopathologia·Muhammad F BhattiRobert H A Coutts
Sep 22, 2017·Frontiers in Microbiology·Ioly Kotta-Loizou, Robert H A Coutts

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