PMID: 2501792Jul 1, 1989Paper

Complete physical map of the human immunoglobulin heavy chain constant region gene complex

Proceedings of the National Academy of Sciences of the United States of America
M H HofkerD W Cox

Abstract

We have found by pulsed-field gel electrophoresis that the human immunoglobulin heavy chain constant region gene complex maps entirely to a 350-kilobase (kb) Mlu I fragment. The enzyme Eag I was used with pulsed-field gel electrophoresis alone and in double digests with Spe I to map the region. C gamma 3, of the C gamma 3-C gamma 1-C psi epsilon 1-C alpha 1 cluster, maps 60 kb to the 3' side of C delta; C gamma 2 of the C gamma 2-C gamma 4-C epsilon-C alpha 2 cluster maps 80 kb to the 3' side of C alpha 1, where C gamma 3 encodes the constant region of the immunoglobulin gamma 3 chain, C gamma 1 encodes the constant region of the immunoglobulin gamma 1 chain, etc. C psi gamma maps 35 kb to the 3' side of C alpha 1 and is in the same transcriptional orientation as the other genes. Although in the cloned DNA many CpG-containing restriction sites were identified, most of these were methylated in peripheral blood leukocytes. The sites that were not methylated were predominantly found in three clusters, or Hpa I tiny fragment islands. One was found on the 5' side of C mu; the other two lie 30 kb to the 3' side of each of the C alpha genes and could indicate the presence of regulatory sequences or genes. A region showing strong linka...Continue Reading

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Citations

Jan 1, 1991·Journal of Clinical Immunology·H SarvasO Mäkelä
Apr 1, 1991·Clinica Chimica Acta; International Journal of Clinical Chemistry·J J van Dongen, I L Wolvers-Tettero
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Jan 21, 2009·Allergology International : Official Journal of the Japanese Society of Allergology·Hiroko SuzukiNaomi Kondo

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