Complete quantification of group A and group B soyasaponins in soybeans

Journal of Agricultural and Food Chemistry
M A BerhowSandra M Duval

Abstract

A combination of high-pressure extraction and preparative chromatography was used to purify the group A and group B soyasaponins from soy germ for use as analytical standards and for use in biological assays. A standardized sample preparation and extraction method was developed for the analysis of phytochemicals found in soy and processed soy products, which is reproducible in other laboratories. The extracts can be analyzed with standard liquid chromatography-mass spectrometry and high-performance liquid chromatography methods to identify and quantitate the group A and group B forms of the soy saponins, as well as the soy isoflavones. Complete saponin analysis of the extracts prepared from soy germ (hypocots), hulls, and cotyledons shows that a significant portion of the saponins is concentrated in the germ. The germ contains nearly all of the group A soyasaponins, while the group B soyasaponins are nearly equally distributed between the germ and the cotyledons. The hulls contain little of either isoflavones or saponins. Whole (full fat) soybeans grown on a tract in central Illinois in 2003 contain approximately 4-6% saponins on a weight basis, of which about one-fifth or less of the total saponin content are group A soyasapon...Continue Reading

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Citations

Apr 20, 2011·Journal of Chemical Ecology·Patrick F DowdEric T Johnson
Jun 19, 2013·Journal of Agricultural and Food Chemistry·Luca ServentiMark L Failla
Jan 15, 2013·Plant Physiology·Doug K Allen, Jamey D Young
Mar 31, 2007·International Journal of Medical Sciences·Manuel T Velasquez, Sam J Bhathena
Aug 25, 2009·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Wei Zhang, David G Popovich
Jan 10, 2012·Journal of Food Science·Satchithanandam EswaranandamSun-Ok Lee
Nov 6, 2012·Food Chemistry·Sun-Lim KimIll-Min Chung
May 31, 2011·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ehsan KarimiSahida Ahmad
Apr 19, 2018·Journal of Food Science and Technology·Hitoshi HorieShigeo Wada
Jul 13, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yuri Mangueira NascimentoJosean Fechine Tavares
Sep 15, 2020·Journal of Animal Science·Sangwoo ParkTofuko Awori Woyengo
Feb 25, 2021·BMC Plant Biology·Hayato SuzukiToshiya Muranaka
May 1, 2021·International Journal of Molecular Sciences·Il-Sup KimWoong-Suk Yang
Oct 25, 2008·Journal of Agricultural and Food Chemistry·Cristina Martinez-VillaluengaElvira Gonzalez de Mejia
May 14, 2008·Journal of Agricultural and Food Chemistry·Wenyi WangElvira Gonzalez de Mejia
Jun 9, 2021·Journal of Agricultural and Food Chemistry·Xiaoyang ZhouJian Zhang
Dec 21, 2006·Journal of Agricultural and Food Chemistry·Sun-Lim KimIll-Min Chung

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