PMID: 9177696May 1, 1997Paper

Complete removal and exchange of sodium dodecyl sulfate bound to soluble and membrane proteins and restoration of their activities, using ceramic hydroxyapatite chromatography

Analytical Biochemistry
M DongF Penin

Abstract

Up to now, removal of sodium dodecyl sulfate (SDS) from proteins in terms of restoration of their activity was an unsolved problem. A general procedure using ceramic hydroxyapatite (HAP) chromatography was developed for the complete removal of SDS bound to soluble or membrane proteins. This procedure involves (i) the binding of the SDS-protein complexes onto the ceramic hydroxyapatite column, (ii) extensive washing of bound proteins with phosphate buffer containing a mild detergent to exchange SDS, (iii) elution of the retained protein by increasing the phosphate concentration. Using this approach, complete exchange of [35S]SDS into a nonionic detergent such as dodecyl maltoside was achieved with a 90-100% protein recovery. The efficiency of protein-bound SDS removal is very likely due to the combined effect of phosphate ions and the hydrophobic tail of nonionic detergent: acting together, they are able to displace SDS molecules from their protein-binding sites. The advantages of this HAP-mediated SDS removal method include high efficiency, rapidity, simplicity and general applicability to a wide variety of detergents and soluble or membrane proteins. Of utmost importance, SDS-treated P-glycoprotein, glutamate dehydrogenase, an...Continue Reading

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Citations

Mar 22, 2014·Protein Science : a Publication of the Protein Society·Zhengrong YangChristie G Brouillette
Nov 3, 2004·Biochimica Et Biophysica Acta·Annela M SeddonPaula J Booth
Feb 10, 2006·Journal of Human Lactation : Official Journal of International Lactation Consultant Association·Sandra Urdaneta HartmannMary K Howett
May 16, 2014·Phlebology·Kurosh Parsi
Aug 8, 2015·Journal of Virology·Pierre FalsonFrançois Penin
Nov 18, 2011·Expert Review of Proteomics·Alan A DoucetteShayla Fitzsimmons
Jul 8, 2017·Biochemical and Biophysical Research Communications·Chuan He, Kouhei Ohnishi
May 8, 1998·Biochemical and Biophysical Research Communications·N C Pomroy, C M Deber
Nov 25, 2000·Biochimica Et Biophysica Acta·M le MaireJ V Moller
Dec 4, 2002·Protein Expression and Purification·Jan SedzikTomitake Tsukihara
Nov 23, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Anna Bodzon-KulakowskaJerzy Silberring

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