Complete sequence analysis and antiviral screening of medicinal plants for human coxsackievirus a16 isolated in Korea

Osong Public Health and Research Perspectives
Jaehyoung SongHyun-Jeong Ko

Abstract

Coxsackievirus A group 16 strain (CVA16) is one of the predominant causative agents of hand, foot, and mouth disease (HFMD). Using a specimen from a male patient with HFMD, we isolated and performed sequencing of the Korean CVA16 strain and compared it with a G10 reference strain. Also, we were investigated the effects of medicinal plant extract on the cytopathic effects (CPE) by CPE reduction assay against Korean CVA16. Phylogenetic analysis showed that the Korean CVA16 isolate belonged to cluster B-1 and was closely related to the strain PM-15765-00 isolated in Malaysia in 2000. The Korean CVA16 isolate showed 73.2% nucleotide identity to the G10 prototype strain and 98.7% nucleotide identity to PM-15765-00. Next, we assessed whether the Korean CVA16 isolate could be used for in vitro screening of antiviral agents to treat HFMD infection. Vero cells infected with the Korean CVA16 isolate showed a cytopathic effect 2 days after the infection, and the treatment of cells with Cornus officinalis, Acer triflorum, Pulsatilla koreana, and Clematis heracleifolia var. davidiana Hemsl extracts exhibited strong antiviral activity against CVA16. Collectively, our work provides potential candidates for the development of vaccine and novel...Continue Reading

References

Feb 1, 1990·Planta medica·M L MartínA Domínguez
Jan 1, 1994·Pharmacology & Therapeutics·L Carrasco
May 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·J W Drake
Sep 25, 1999·The New England Journal of Medicine·M HoS R Shih
Dec 26, 2001·Antiviral Research·Harley A Rotbart
Aug 13, 2003·Journal of Applied Microbiology·S A A Jassim, M A Naji
Nov 1, 1955·Proceedings of the Society for Experimental Biology and Medicine·G M SICKLESH PLAGER
Mar 12, 2004·The Pediatric Infectious Disease Journal·Chung-Yi WangLi-Min Huang
Oct 12, 2004·Archives of Pharmacal Research·Yong KimByung-Zun Ahn
May 10, 2007·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Jun 9, 2009·Transactions of the Royal Society of Tropical Medicine and Hygiene·Chin-Chou WangMeng-Chih Lin
Nov 27, 2009·Medical Microbiology and Immunology·Holger F RabenauHans Wilhelm Doerr
Jan 6, 2010·Indian Journal of Dermatology·Nilendu SarmaRajib Malakar
Mar 2, 2011·Korean journal of pediatrics·Cheol Soon ChoiJin Han Kang
Mar 7, 2014·Biomolecules & Therapeutics·Jaehyoung SongHyun-Jeong Ko
Mar 14, 2014·Expert Review of Vaccines·Jing-Xin LiFeng-Cai Zhu

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Citations

Dec 23, 2015·Viruses·Zhi-Chao ZhuangHong-Ling Wen
May 3, 2016·Journal of Ethnopharmacology·Wu BiPeigen Xiao
Nov 4, 2016·Food and Environmental Virology·Dong Joo Seo, Changsun Choi
Feb 17, 2021·Phytotherapy Research : PTR·Sofi Imtiyaz AliShowkat Ul Nabi

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Datasets Mentioned

BETA
JX839965

Methods Mentioned

BETA
PCR

Software Mentioned

MEGA
CLUSTAL W
Basic Local Alignment Search Tool ( BLAST )
Graphpad P

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