Complete sequence and structural organization of pFL5 and pFL7, two cryptic plasmids from Bacillus licheniformis

C PariniG Ricci


The complete nucleotide sequences of two plasmids, pFL5 and pFL7, isolated from soil bacteria, Bacillus licheniformis FL5 and FL7, have been determined. The plasmids pFL5 and pFL7 were analyzed and found to be 9150 and 7853 bp in size with a G+C content of 41.0 and 43.6 mol%, respectively. Computer assisted analysis of sequence data revealed 11 possible ORFs in pFL5, four of which could be assigned no function from homology searches. Instead, eight putative ORFs were identified in pFL7, two of which appeared to have no biological function. All the ORFs were preceded by a ribosome binding site. The ORFs 9.5 and 6.7, each of 340 amino acids, were postulated to encode a replication protein similar to known replication proteins of rolling circle replicons, particularly those of the pC194 family. The structural organization of the two pFL plasmids is similar to the pTA plasmids family, with only a few putative coding regions that cannot be attributed to these plasmid backbone genes. In contrast to pTA plasmids, the majority of the genes have an orientation of transcription opposite to the direction of replication. The identified probable sso sequences seem to belong to a different group of those found in Bacillus plasmids; in fact, ...Continue Reading


Dec 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·F SangerA R Coulson
Mar 1, 1977·Journal of Bacteriology·T TanakaK Sakaguchi
Aug 1, 1977·Journal of Bacteriology·T Tanaka, T Koshikawa
Jul 1, 1992·Journal of Bacteriology·J P Mueller, A L Sonenshein
Jan 1, 1989·Annual Review of Microbiology·R P Novick
May 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·H YamagataS Udaka
Nov 1, 1987·Molecular & General Genetics : MGG·M MuraiY Oshima
Oct 10, 1995·Proceedings of the National Academy of Sciences of the United States of America·C GuilhotJ Beckwith
Feb 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·J C BardwellJ Beckwith
Jan 1, 1996·Journal of Bacteriology·P ZawadzkiF M Cohan
Feb 20, 1996·Proceedings of the National Academy of Sciences of the United States of America·M Perego, J A Hoch
Sep 2, 1998·Proceedings of the National Academy of Sciences of the United States of America·M G KramerS A Khan
Mar 9, 2000·Trends in Microbiology·F de la Cruz, J Davies
Jun 1, 2000·Nature·H OchmanE A Groisman
Aug 10, 2000·Molecular Microbiology·K GerdesR Bugge Jensen
Aug 10, 2000·Nucleic Acids Research·M FrohmeA P de Souza
Aug 11, 2000·Molecular and Cellular Biology·A ZiemienowiczB Hohn
Nov 15, 2000·Genome Research·S Garcia-VallvéJ Palau
Oct 7, 2004·Applied and Environmental Microbiology·Dongru QiuMitsuhiro Itaya
Dec 1, 1983·Applied and Environmental Microbiology·K YoshimuraY Oshima

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