PMID: 6170937Sep 25, 1981Paper

Complete sequence of an immunoglobulin mRNA using specific priming and the dideoxynucleotide method of RNA sequencing

Nucleic Acids Research
P H HamlynC Milstein

Abstract

The complete sequence of the mouse immunoglobulin kappa light chain MOPC 21 messenger RNA has been determined using a chain termination method and chemically synthesised deoxyoligonucleotides to initiate the synthesis of a DNA molecule complementary to the mRNA template. Five such oligonucleotide primers have been used for the sequence analysis of this messenger RNA. The approach is excellent for comparative studies of mouse k-chain mRNAs because they can be made on impure mRNA preparations. The MOPC 21 light chain mRNA is 943 nucleotides in length excluding the poly(A) region. An unexpected finding was that there are only three bases in the 5' non-coding region and its significance in terms of ribosome binding is discussed; 87 code for the precursor or leader sequence of the protein, 642 for the mature protein and 211 for the 3' non-coding region. The codons for the precursor region allows the previously undetermined amino acid sequence to be predicted. In common with other precursor regions a high proportion of the predicted amino acids are hydrophobic.

References

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Citations

Oct 12, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·C Milstein
Apr 1, 1989·Molecular & General Genetics : MGG·A S Byström, G R Fink
Sep 13, 1996·Journal of Immunological Methods·S M KipriyanovG Moldenhauer
Oct 1, 1988·Molecular Immunology·W L CarrollS Levy
Dec 28, 1995·Brain Research. Molecular Brain Research·K AsakuraM Rodriguez
Feb 1, 1996·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·E M Denovan-WrightJ M Wright
May 30, 1988·Gene·L I González-VillaseñorD A Powers
Jun 1, 1993·Insect Biochemistry and Molecular Biology·J F KoenerR Feyereisen
Mar 1, 1994·Insect Biochemistry and Molecular Biology·A B Mulnix, P E Dunn
Mar 1, 1992·Biologicals : Journal of the International Association of Biological Standardization·G ContrerasL Wu
Jun 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·S CabillyH L Heyneker
Oct 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·M NishiT Honjo
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·S BergströmJ Swanson
Jun 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·R BaccalaS Avrameas
Jul 10, 1987·Nucleic Acids Research·F J GrantW Kindsvogel
Jan 1, 1984·Annales d'immunologie·C Berek
Dec 9, 2004·Immunology Letters·Jennifer K JohnsonDavid E Lenz
Oct 1, 1988·Immunological Reviews·C Berek, C Milstein

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