Complete Sequence of Succinamopine Ti-Plasmid pTiEU6 Reveals Its Evolutionary Relatedness with Nopaline-Type Ti-Plasmids

Genome Biology and Evolution
Shuai ShaoP J Hooykaas

Abstract

Agrobacterium tumefaciens is the etiological agent of plant crown gall disease, which is induced by the delivery of a set of oncogenic genes into plant cells from its tumor-inducing (Ti) plasmid. Here we present the first complete sequence of a succinamopine-type Ti-plasmid. Plasmid pTiEU6 is comprised of 176,375 bp with an overall GC content of 56.1% and 195 putative protein-coding sequences could be identified. This Ti-plasmid is most closely related to nopaline-type Ti-plasmids. It contains a single T-region which is somewhat smaller than that of the nopaline-type Ti-plasmids and in which the gene for nopaline synthesis is replaced by a gene (sus) for succinamopine synthesis. Also in pTiEU6 the nopaline catabolic genes are replaced by genes for succinamopine catabolism. In order to trace the evolutionary origin of pTiEU6, we sequenced six nopaline Ti-plasmids to enlarge the scope for comparison to this class of plasmids. Average nucleotide identity analysis revealed that pTiEU6 was most closely related to nopaline Ti-plasmids pTiT37 and pTiSAKURA. In line with this traces of several transposable elements were present in all the nopaline Ti plasmids and in pTiEU6, but one specific transposable element insertion, that of a cop...Continue Reading

References

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Citations

Jan 1, 2021·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Rosa PadillaIsabelle Kerzaon
Apr 8, 2021·Plant Molecular Biology·Léon Otten
Jul 1, 2021·Phytochemistry·Tatiana Matveeva, Léon Otten

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Methods Mentioned

BETA
transgenic
PCRs
electrophoresis

Software Mentioned

BlastN
BaseClear
kSNP3
eggNOG
Kchooser
IS
WebLogo
Glimmer
IGS Prokaryotic Annotation Pipeline
Perl script

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