Complete sequence of the floR-carrying multiresistance plasmid pAB5S9 from freshwater Aeromonas bestiarum

The Journal of Antimicrobial Chemotherapy
Laurence GordonEtienne Giraud

Abstract

A multiresistant Aeromonas bestiarum strain, shown to be persistent and spreading in a freshwater stream, was investigated for the presence, location and organization of antimicrobial resistance genes. The plasmid pAB5S9 was transferred by electroporation into Escherichia coli TG1. The resistance phenotype mediated by pAB5S9 was determined. Moreover, the plasmid was sequenced completely and analysed for its structure and organization of reading frames. Plasmid pAB5S9 mediated resistances to phenicols, sulphonamides, streptomycin and tetracycline. The analysis of the 24.7 kb sequence revealed the presence of 20 predicted coding sequences (CDSs), which included the floR, sul2 and strA-strB resistance genes and a tetR-tet(Y) determinant. Approximately 7.5 kb of pAB5S9 showed 100% nucleotide sequence identity to three non-contiguous segments of the SXT element of Vibrio cholerae. Regions identical to SXT comprised the floR gene, flanked upstream by a complete and downstream by a truncated ISCR2 element, and the region of the sul2 and strA-strB genes. Other CDSs of pAB5S9 related to plasmid replication and partitioning, metabolic and gene regulation functions as well as conjugative transfer showed homology to sequences from diverse ...Continue Reading

References

Oct 1, 1984·Journal of Clinical Microbiology·S Takahashi, Y Nagano
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
May 7, 1998·Chemosphere·B Halling-SørensenS E Jørgensen
Aug 10, 2000·The Journal of Antimicrobial Chemotherapy·M Goñi-UrrizaC Quentin
Dec 26, 2001·The Journal of Antimicrobial Chemotherapy·Benoît DoubletAxel Cloeckaert
Nov 13, 2004·FEMS Microbiology Reviews·Stefan SchwarzAxel Cloeckaert
Apr 28, 2005·Antimicrobial Agents and Chemotherapy·Benoît DoubletAxel Cloeckaert
Jun 28, 2005·The Journal of Antimicrobial Chemotherapy·Tomasz HauschildStefan Schwarz
Apr 26, 2006·The Journal of Antimicrobial Chemotherapy·Kristina KadlecStefan Schwarz
Jun 9, 2006·Microbiology and Molecular Biology Reviews : MMBR·Mark A TolemanTimothy R Walsh
Aug 18, 2006·Environmental Microbiology·Ramunas StepanauskasJ V McArthur
Sep 1, 2006·Current Opinion in Microbiology·Timothy R Walsh
Sep 5, 2006·Applied and Environmental Microbiology·Jennifer R HuddlestonRandall M Jeter
Sep 19, 2006·Journal of Bacteriology·Rekha SeshadriJohn F Heidelberg
Dec 22, 2006·PloS One·Maria C AlmeidaAndrej A Romanovsky

❮ Previous
Next ❯

Citations

Sep 13, 2008·The Journal of Antimicrobial Chemotherapy·Corinna KehrenbergStefan Schwarz
Jul 14, 2010·Antimicrobial Agents and Chemotherapy·Kathleen M SchleinitzSabine Kleinsteuber
Sep 14, 2010·Drug Resistance Updates : Reviews and Commentaries in Antimicrobial and Anticancer Chemotherapy·Maria S Ramirez, Marcelo E Tolmasky
Jan 31, 2016·Journal of Health, Population, and Nutrition·Maanasa BhaskarJharna Mandal
May 29, 2013·Environmental Microbiology·Felipe C CabelloAlejandro H Buschmann
Mar 25, 2015·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Tatiana I LobovaVladimir Ye Repin
Jun 16, 2015·Frontiers in Microbiology·Marta Piotrowska, Magdalena Popowska
May 10, 2015·Marine Pollution Bulletin·Claudio D MirandaGerardo González-Rocha
Nov 10, 2018·Antimicrobial Resistance and Infection Control·Junwan LuQiyu Bao
Sep 8, 2010·Expert Review of Anti-infective Therapy·Daniel Curcio
Dec 13, 2019·Frontiers in Cellular and Infection Microbiology·Hossam AbdelhamedMark L Lawrence
May 12, 2019·Probiotics and Antimicrobial Proteins·Carolina RamírezJaime Romero
May 10, 2020·Archives of Microbiology·Lucas David Rodrigues Dos SantosEliana Guedes Stehling
Oct 12, 2020·Annals of the New York Academy of Sciences·Antony T VincentSteve J Charette
May 8, 2020·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·João Pedro Rueda FurlanEliana Guedes Stehling
Jan 27, 2018·Microbiology Spectrum·Ron A Miller, Heather Harbottle
Apr 28, 2010·Antimicrobial Agents and Chemotherapy·C García-de-la-MàriaUNKNOWN Hospital Clínic Experimental Endocarditis Study Group
May 17, 2021·Ecotoxicology·Lateef Babatunde SalamOlukayode Oladipo Amund

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.