Complete sequences of KPC-2-encoding plasmid p628-KPC and CTX-M-55-encoding p628-CTXM coexisted in Klebsiella pneumoniae

Frontiers in Microbiology
Li WangDongsheng Zhou

Abstract

A carbapenem-resistant Klebsiella pneumoniae strain 628 was isolated from a human case of intracranial infection in a Chinese teaching hospital. Strain 628 produces KPC-2 and CTX-M-55 encoded by two different conjugative plasmids, i.e., the IncFIIK plasmid p628-KPC and the IncI1 plasmid p628-CTXM respectively. bla KPC-2 is captured by a Tn1722-based unit transposon with a linear structure. ΔTn3-ISKpn27-bla KPC-2-ΔISKpn6-ΔTn1722 and this transposon together with a mercury resistance (mer) gene locus constitutes a 34 kb acquired drug-resistance region. bla KPC-2 has two transcription starts (nucleotides G and C located at 39 and 250 bp upstream of its coding region respectively) which correspond to two promoters, i.e., the intrinsic P1 and the upstream ISKpn27/Tn3-provided P2 with the core -35/-10 elements TAATCC/TTACAT and TTGACA/AATAAT respectively. bla CTX-M-55 is mobilized in an ISEcp1-bla CTX-M-55-Δorf477 transposition unit and appears to be the sole drug-resistant determinant in p628-CTXM. bla CTX-M-55 possesses a single transcription start (nucleotides G located at 116 bp upstream of its coding region) corresponding to the ISEcp1-provided P1 promoter with the core -35/-10 element TTGAAA/TACAAT. All the above detected promo...Continue Reading

References

Dec 31, 2005·Nucleic Acids Research·P SiguierM Chandler
Mar 30, 2006·Antimicrobial Agents and Chemotherapy·Marie-Frédérique LartiguePatrice Nordmann
Jun 15, 2007·Antimicrobial Agents and Chemotherapy·Azita LeavittYehuda Carmeli
Jan 30, 2008·Antimicrobial Agents and Chemotherapy·Thierry NaasPatrice Nordmann
Feb 26, 2008·Applied and Environmental Microbiology·Jeremy A FrankGary J Olsen
Sep 10, 2008·Plasmid·Adam P RobertsDouglas E Berg
Nov 26, 2008·The Journal of Antimicrobial Chemotherapy·Gisele PeiranoMarise D Asensi
Jan 1, 2010·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Maria SouliHelen Giamarellou
Mar 31, 2010·Antimicrobial Agents and Chemotherapy·Azita LeavittShiri Navon-Venezia
Apr 28, 2010·Antimicrobial Agents and Chemotherapy·Zhiyong ZongJonathan R Iredell
Jul 3, 2010·Plasmid·Gen-Ichi SampeiTeruya Komano
Jul 28, 2010·Antimicrobial Agents and Chemotherapy·Brandon KitchelJean B Patel
Dec 15, 2010·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·G LiX Jiang
Aug 17, 2011·Antimicrobial Agents and Chemotherapy·Gaelle CuzonPatrice Nordmann
Dec 29, 2011·Antimicrobial Agents and Chemotherapy·Liang ChenBarry N Kreiswirth
Jun 27, 2012·Antimicrobial Agents and Chemotherapy·Thierry NaasPatrice Nordmann
Oct 20, 2012·Veterinary Microbiology·P L HoK H Chow
Apr 10, 2013·The Journal of Antimicrobial Chemotherapy·Thierry NaasPatrice Nordmann
Aug 24, 2013·The Lancet Infectious Diseases·L Silvia Munoz-PriceJohn P Quinn
Oct 2, 2013·Proceedings of the National Academy of Sciences of the United States of America·Kathryn E HoltStephen Baker
Oct 15, 2013·The Journal of Antimicrobial Chemotherapy·Ying-Tsong ChenL Kristopher Siu
Feb 26, 2014·Antimicrobial Agents and Chemotherapy·Paula Juliana Pérez-ChaparroJohn Anthony McCulloch
Apr 20, 2014·Diagnostic Microbiology and Infectious Disease·Inna ChmelnitskyYehuda Carmeli
May 1, 2015·Frontiers in Microbiology·Zhenhong ChenZhe Yin

❮ Previous
Next ❯

Citations

Jan 18, 2017·Proceedings of the National Academy of Sciences of the United States of America·Gustavo C CerqueiraWilliam P Hanage
Nov 2, 2017·The Journal of Antimicrobial Chemotherapy·Beiwen ZhengLanjuan Li
Nov 16, 2017·Future Microbiology·Lizhi MaDongsheng Zhou
Aug 12, 2017·Future Microbiology·Defu ZhangDongsheng Zhou
May 10, 2020·Biomolecules·Maria Soledad RamirezMarcelo E Tolmasky
Mar 5, 2020·The Journal of Antimicrobial Chemotherapy·Zhenpeng LiFang Bai
Sep 10, 2020·Infection and Drug Resistance·Xiufeng ZhangZhenling Zeng
Feb 9, 2021·Journal of Basic Microbiology·Hongchao ChenYanjun Zhang
Oct 5, 2021·Journal of Global Antimicrobial Resistance·Tran Dieu LinhTran Huy Hoang
Jun 5, 2020·Ecotoxicology and Environmental Safety·João Pedro Rueda FurlanEliana Guedes Stehling

❮ Previous
Next ❯

Datasets Mentioned

BETA
KP987218
KP987217
CP000649
FJ628167
KF623109
KC405622

Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

BLASTP
BLASTN
CLUSTALW
Velvet
ISfinder

Related Concepts

Related Feeds

Carbapenems (ASM)

Carbapenems are members of the beta lactam class of antibiotics and are used for the treatment of severe or high-risk bacterial infections. Discover the latest research on carbapenems here.

Carbapenems

Carbapenems are members of the beta lactam class of antibiotics and are used for the treatment of severe or high-risk bacterial infections. Discover the latest research on carbapenems here.