Complete structures of Bordetella bronchiseptica and Bordetella parapertussis lipopolysaccharides

The Journal of Biological Chemistry
Andrew PrestonEvgeny Vinogradov

Abstract

The structures of the lipopolysaccharide (LPS) core and O antigen of Bordetella bronchiseptica and Bordetella parapertussis are known, but how these two regions are linked to each other had not been determined. We have studied LPS from several strains of these microorganisms to determine the complete carbohydrate structure of the LPS. LPS was analyzed using different chemical degradations, NMR spectroscopy, and mass spectrometry. This identified a novel pentasaccharide fragment that links the O chain to the core in all the LPS studied. In addition, although the O chain of these bacteria was reported as a homopolymer of 1,4-linked 2,3-diacetamido-2,3-dideoxy-alpha-galacturonic acid, we discovered that the polymer contains several amidated uronic acids, the number of which varies between strains. These new data describe the complete structure of the LPS carbohydrate backbone for both Bordetella species and help to explain the complex genetics of LPS biosynthesis in these bacteria.

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Citations

Dec 25, 2012·Carbohydrate Research·Joanna Kubler-Kielb, Evgeny Vinogradov
Apr 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·John B RobbinsRachel Schneerson
Mar 4, 2011·Proceedings of the National Academy of Sciences of the United States of America·Joanna Kubler-KielbRachel Schneerson
Feb 21, 2008·Infection and Immunity·Elizabeth M GoebelEric T Harvill
Sep 2, 2010·Microbiology and Molecular Biology Reviews : MMBR·Leslie CuthbertsonChris Whitfield
Mar 13, 2014·Nature Reviews. Microbiology·Jeffrey A MelvinPeggy A Cotter
Dec 8, 2015·Microbiological Research·M Fingermann, D Hozbor
Sep 17, 2013·International Journal of Medical Microbiology : IJMM·Rosanna E B YoungDerek W Hood
Feb 22, 2014·Proceedings of the National Academy of Sciences of the United States of America·John B RobbinsJoseph Shiloach
Aug 28, 2009·Innate Immunity·Jerry D KingJoseph S Lam
Aug 2, 2014·Expert Review of Vaccines·Jeroen GeurtsenGermie P J M van den Dobbelsteen
Apr 6, 2012·Carbohydrate Research·Laura K Greenfield, Chris Whitfield
Apr 1, 2016·Clinical Microbiology Reviews·Paul E KilgoreHeinz-Josef Schmitt
Jun 14, 2019·Journal of the American Society for Mass Spectrometry·Adriana C CasabuonoAlicia S Couto

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