Complete transcriptional map of yeast chromosome XI in different life conditions

Journal of Molecular Biology
G F RichardBernard Dujon

Abstract

Systematic sequencing of the genome of Saccharomyces cerevisiae has demonstrated the existence of many novel genes, whose functions need to be studied. Entire chromosome sequences also offer the possibility to examine functional properties of the genome at a higher hierarchical level than the genes themselves. We used ordered DNA fragments of chromosome XI to systematically probe yeast DNA and total RNA extracted from MAT a, MAT alpha and diploid cells grown under three different conditions. Taking into account transcript sizes and uniqueness of probes, we attributed 94 transcripts to sequence-predicted open reading frames (ORFs) or tRNA genes; another 83 being tentatively assigned. The remaining 187 ORFs on chromosome XI do not correspond to transcripts detected under our conditions. More than 80% of transcripts are constitutively expressed, others are regulated by medium composition or cell type, the most frequent regulations being determined by carbon source (glycerol/glucose) or rich versus synthetic medium. Moreover, we show that transcript levels and regulation patterns are not statistically different between ORFs of unknown function, which constitute ca. 40% of the total, and previously identified genes (ca. 30%) or thei...Continue Reading

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Citations

Jan 7, 1998·Nature Biotechnology·L WodickaD J Lockhart
Aug 15, 2002·Nucleic Acids Research·Emmanuelle FabreGuy-Franck Richard
Jan 8, 2004·Genome Biology·Evan H Hurowitz, Patrick O Brown
Apr 17, 1999·Yeast·R J PlantaJ Winderickx
Sep 24, 2005·Molecular and Cellular Biochemistry·Mrinal Kanti GhoshVani Brahmachari
Dec 11, 1999·Yeast·R NiedenthalJ H Hegemann
Oct 2, 2002·Journal of Biomolecular Structure & Dynamics·Mrinal Kanti GhoshRamesh Chandra
Jul 31, 1999·The Journal of Biological Chemistry·L PalmieriJ E Walker
Feb 20, 1998·Current Opinion in Genetics & Development·E A Winzeler, R W Davis
Feb 15, 2000·Research in Microbiology·G F RichardB Dujon
May 1, 1996·Current Biology : CB·M Johnston

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