Complete unilateral ureteral obstruction in the fetal lamb. Part II: Long-term outcomes of renal tissue development

The Journal of Urology
Pierre-Yves MurePierre Mouriquand

Abstract

We analyzed the dynamics of the renal tissue response to experimental fetal urinary flow impairment concerning renal morphology, extracellular matrix composition, regulators of connective tissue degradation and PAX2 protein expression. A total of 26 fetal lambs underwent surgical unilateral ureteral obstruction at 90 days of gestation and 14 twin matched animals served as controls. Kidneys were harvested 10, 20 and 40 days after the prior procedure in groups 1 to 3, respectively and in 1-month-old lambs (group 4). Morphological analysis was done using light microscopy. Picrosirius red staining was used to evaluate the area occupied by extracellular matrix components. Collagen I, III and IV, alpha-smooth muscle actin, MMP-1, 2 and 9, TIMP-1 and 2 and PAX2 protein were assessed using immunochemistry. All obstructed kidneys were hydronephrotic without dysplasia. Hypoplasia resulting from a decreased NGG was observed. The inflammatory response to obstruction was poor in fetal obstructed kidneys. From 10 days after obstruction interstitial fibrosis was noted and confirmed by an increase in picrosirius red staining. In obstructed kidneys immunochemistry showed an increase in collagen deposition beginning from the papillae and extendi...Continue Reading

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Citations

Sep 6, 2012·International Journal of Molecular Sciences·Tian-Biao ZhouYan-Jun Zhao
Jan 11, 2012·Renal Failure·Tian-Biao ZhouWei-Fang Huang
Aug 5, 2010·International Journal of Experimental Pathology·Julie KleinJoost P Schanstra
Nov 10, 2012·Journal of Receptor and Signal Transduction Research·Tian-Biao Zhou

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