Completed Genomic Sequence of Bacillus thuringiensis HER1410 Reveals a Cry-Containing Chromosome, Two Megaplasmids, and an Integrative Plasmidial Prophage.

G3 : Genes - Genomes - Genetics
A. LechugaM. Redrejo-Rodriguez

Abstract

Bacillus thuringiensis is the most used biopesticide in agriculture. Its entomopathogenic capacity stems from the possession of plasmid-borne insecticidal crystal genes (cry), traditionally used as discriminant taxonomic feature for that species. As such, crystal and plasmid identification are key to the characterization of this species. To date, about 600 B. thuringiensis genomes have been reported, but less than 5% have been completed, while the other draft genomes are incomplete, hindering full plasmid delineation. Here we present the complete genome of Bacillus thuringiensis HER1410, a strain closely related to B. thuringiensis entomocidus and a known host for a variety of Bacillus phages. The combination of short and long-read techniques allowed fully resolving the genome and delineation of three plasmids. This enabled the accurate detection of an unusual location of a unique cry gene, cry1Ba4, located in a genomic island near the chromosome replication origin. Two megaplasmids, pLUSID1 and pLUSID2 could be delineated: pLUSID1 (368 kb), a likely conjugative plasmid involved in virulence, and pLUSID2 (156 kb) potentially related to the sporulation process. A smaller plasmidial prophage pLUSID3, with a dual lifestyle whose i...Continue Reading

References

Apr 1, 1983·Journal of Bacteriology·J W KronstadH R Whiteley
Feb 1, 1993·Journal of Bacteriology·C R Carlson, A B Kolstø
Dec 3, 1999·Applied and Environmental Microbiology·B M PrüssS Scherer
Sep 27, 2002·Applied and Environmental Microbiology·Colin BerryJulian Parkhill
Oct 9, 2002·Genome Research·Jason E StajichEwan Birney
Dec 13, 2005·Structure·Pasi Antero LaurinmäkiSarah Jane Butcher
Dec 31, 2005·Nucleic Acids Research·P SiguierM Chandler
Aug 4, 2006·Journal of Bacteriology·Ausra GaidelyteDennis H Bamford
Oct 13, 2006·Current Microbiology·Sheila NathanNor M Mahadi
Feb 10, 2007·Journal of Microbiological Methods·Rimantas DaugelaviciusDennis H Bamford
Jun 23, 2009·Genome Research·Martin KrzywinskiMarco A Marra
Feb 1, 2011·Bioinformatics·Mitchell J SullivanScott A Beatson
Apr 18, 2012·Journal of Computational Biology : a Journal of Computational Molecular Cell Biology·Anton BankevichPavel A Pevzner
May 26, 2012·International Journal of Cardiology·Alexandra RowellGemma A Figtree
Feb 21, 2013·Bioinformatics·Alexey GurevichGlenn Tesler
May 25, 2013·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Ben Raymond, Michael B Bonsall
Apr 4, 2014·Bioinformatics·Anthony M BolgerBjoern Usadel
Apr 8, 2014·Journal of Bacteriology·Lynne S CairnsNicola R Stanley-Wall
May 6, 2014·Applied and Environmental Microbiology·Annika Gillis, Jacques Mahillon
Sep 3, 2014·Journal of Microbiological Methods·Annika Gillis, Jacques Mahillon
Nov 6, 2014·Molecular Biology and Evolution·Lam-Tung NguyenBui Quang Minh
Dec 24, 2014·Nature Reviews. Microbiology·Mart Krupovic, Eugene V Koonin
Jun 24, 2015·Bioinformatics·Ryan R WickKathryn E Holt
Jun 25, 2015·Microbiology Spectrum·Vicki AdamsJulian I Rood
Jul 23, 2015·Bioinformatics·Andrew J PageJulian Parkhill
May 5, 2016·Nucleic Acids Research·David ArndtDavid S Wishart
Jun 28, 2016·Nucleic Acids Research·Tatiana TatusovaJames Ostell
Jul 29, 2016·Nucleic Acids Research·Mónica Berjón-OteroModesto Redrejo-Rodríguez
Oct 30, 2016·Research in Microbiology·Annika GillisJacques Mahillon
Nov 5, 2016·Research in Microbiology·Alexandre BolotinAlexei Sorokin
Feb 17, 2017·Antonie van Leeuwenhoek·Seok-Hwan YoonJongsik Chun
May 2, 2017·Molecular Biology and Evolution·Jaime Huerta-CepasPeer Bork
May 5, 2017·Nucleic Acids Research·Claire BertelliFiona Sl Brinkman
May 10, 2017·Nature Methods·Subha KalyaanamoorthyLars S Jermiin

❮ Previous
Next ❯

Citations

Dec 15, 2020·Applied and Environmental Microbiology·Nancy FayadMireille Kallassy Awad
Jul 20, 2021·Frontiers in Microbiology·Stefani Díaz-ValerioHeiko Liesegang

❮ Previous
Next ❯

Datasets Mentioned

BETA
PRJNA613019
CP011350.1
AP014864.1

Methods Mentioned

BETA
electrophoresis

Software Mentioned

Phaster
Porechop
BLASTn
BLASTp
Circos
ANI Calculator
IslandViewer
FigTree
Bandage
eggNOG mapper

Related Concepts

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.