Complex antigen presentation pathway for an HLA-A*0201-restricted epitope from Chikungunya 6K protein

PLoS Neglected Tropical Diseases
Elena LorenteDaniel Lopez

Abstract

The adaptive cytotoxic T lymphocyte (CTL)-mediated immune response is critical for clearance of many viral infections. These CTL recognize naturally processed short viral antigenic peptides bound to human leukocyte antigen (HLA) class I molecules on the surface of infected cells. This specific recognition allows the killing of virus-infected cells. The T cell immune T cell response to Chikungunya virus (CHIKV), a mosquito-borne Alphavirus of the Togaviridae family responsible for severe musculoskeletal disorders, has not been fully defined; nonetheless, the importance of HLA class I-restricted immune response in this virus has been hypothesized. By infection of HLA-A*0201-transgenic mice with a recombinant vaccinia virus that encodes the CHIKV structural polyprotein (rVACV-CHIKV), we identified the first human T cell epitopes from CHIKV. These three novel 6K transmembrane protein-derived epitopes are presented by the common HLA class I molecule, HLA-A*0201. One of these epitopes is processed and presented via a complex pathway that involves proteases from different subcellular locations. Specific chemical inhibitors blocked these events in rVACV-CHIKV-infected cells. Our data have implications not only for the identification of...Continue Reading

References

Jan 1, 1988·Methods in Enzymology·D S Auld
Nov 1, 1969·The American Journal of Tropical Medicine and Hygiene·S B HalsteadS Udomsakdi
Jul 1, 1984·Journal of Virology·B SimizuT Ogata
Jan 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·H K Ziegler, E R Unanue
Jan 15, 1982·Journal of Molecular Biology·C M Rice, J H Strauss
Jan 1, 1994·Methods in Enzymology·N A Thornberry
Jan 13, 2000·Immunological Reviews·I A YorkK L Rock
May 9, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·D LópezM Del Val
May 29, 2000·European Journal of Biochemistry·L SchomburgK Bauer
Mar 23, 2001·Nature Immunology·L StoltzeH Schild
Jun 22, 2002·Science·Andreas WeihofenBruno Martoglio
Apr 23, 2003·Microbes and Infection·Georg LautschamNeil Blake
Jun 18, 2003·The Journal of Biological Chemistry·Cécile ContinJulie Déchanet-Merville
Jan 1, 1955·Transactions of the Royal Society of Tropical Medicine and Hygiene·W H LUMSDEN
Jun 30, 2004·Nature Immunology·Kenneth L RockAlfred L Goldberg
Feb 5, 2005·Transactions of the Royal Society of Tropical Medicine and Hygiene·Kanti LarasAndrew L Corwin
Jun 6, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Neeraj TiwariFrank Momburg
Aug 19, 2007·The Journal of General Virology·Ann M Powers, Christopher H Logue
May 3, 2008·Methods in Molecular Biology·Mathias M SchulerStefan Stevanovikć
Sep 8, 2009·Virology·Maxime SolignatChristian Devaux
Jan 1, 1946·The Journal of General Physiology·M KUNITZ

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Methods Mentioned

BETA
PCR
flow cytometry
transgenic

Software Mentioned

FlowJo
SYFPEITHI

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