Complex coacervation-based loading and tunable release of a cationic protein from monodisperse glycosaminoglycan microgels

Soft Matter
Carl C L SchuurmansT Vermonden

Abstract

Glycosaminoglycans (GAGs) are of interest for biomedical applications because of their ability to retain proteins (e.g. growth factors) involved in cell-to-cell signaling processes. In this study, the potential of GAG-based microgels for protein delivery and their protein release kinetics upon encapsulation in hydrogel scaffolds were investigated. Monodisperse hyaluronic acid methacrylate (HAMA) and chondroitin sulfate methacrylate (CSMA) micro-hydrogel spheres (diameters 500-700 μm), were used to study the absorption of a cationic model protein (lysozyme), microgel (de)swelling, intra-gel lysozyme distribution and its diffusion coefficient in the microgels dispersed in buffers (pH 7.4) of varying ionic strengths. Upon incubation in 20 mM buffer, lysozyme was absorbed up to 3 and 4 mg mg-1 dry microspheres for HAMA and CSMA microgels respectively, with loading efficiencies up to 100%. Binding stoichiometries of disaccharide : lysozyme (10.2 : 1 and 7.5 : 1 for HAMA and CSMA, respectively) were similar to those for GAG-lysozyme complex coacervates based on soluble GAGs found in literature. Complex coacervates inside GAG microgels were also formed in buffers of higher ionic strengths as opposed to GAG-lysozyme systems based on so...Continue Reading

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Citations

Feb 18, 2020·Macromolecular Bioscience·Jacobus A W JongCornelus F van Nostrum
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Methods Mentioned

BETA
fluorescence microscopy
Fluorescence Recovery After Photobleaching
fluorescence after photobleaching
confocal microscopy

Software Mentioned

GraphPad Prism
NIS
elements
R
FRAPanalyzer

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