Complex Coacervation of Milk Proteins with Sodium Alginate

Journal of Agricultural and Food Chemistry
Elham Ghorbani GorjiSara Ghorbani Gorji

Abstract

Beta-lactoglobulin (BLG) and bovine serum albumin (BSA) coacervate formation with sodium alginate (ALG) was investigated by turbidimetric analysis, zeta potential, particle size, viscosity, transmission electron microscopy (TEM) and isothermal titration calorimetric (ITC) measurements as a function of pH (1.0-7.0) and protein/alginate mixing ratio (1:1, 1.5:1, 2:1, 1:0, and 0:1 wt.). Critical pH values of phase transitions for BSA-ALG complexes (pHC, pHφ1, and pHφφ2) representing the formation of soluble and insoluble complexes of a protein-ALG mixture (2:1) at pH 4.8, 4.2, and 1.8, respectively. In the case of BLG-ALG, critical pH values (pHC, pHφ1, and pHφ2) were found to be 4.8, 4.2, and 1.6, respectively. The pHopt values, expressed by the highest optical density, were pH 2.8 for BSA-ALG and 2.4 for BLG-ALG. TEM and zeta-potential results showed that maximum coacervate formation occurred at pH 4.2 for both protein-polysaccharide solutions. The interaction between BLG-ALG and BSA-ALG was spontaneously exothermic at pH 4.2 according to ITC measurements. The findings of this study provide insights to a thorough understanding about the nature of interactions between milk proteins and ALG and formulate new applications for food,...Continue Reading

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Citations

May 5, 2020·Small·Whitney C Blocher McTigue, Sarah L Perry
Dec 5, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Wenxiao JiaoWeijun Chen
Aug 25, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Guiying HuangQingrong Huang
Jan 9, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Yushuang YangMutai Bao

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