Complex Effects of 24:1 Sphingolipids in Membranes Containing Dioleoylphosphatidylcholine and Cholesterol

Langmuir : the ACS Journal of Surfaces and Colloids
Aritz B García-ArribasFélix M Goñi

Abstract

The effects of C24:1 sphingolipids have been tested in phospholipid bilayers containing cholesterol. Confocal microscopy, differential scanning calorimetry, and atomic force microscopy imaging and force curves have been used. More precisely, the effects of C24:1 ceramide (nervonoyl ceramide, nCer) were evaluated and compared to those of C16:0 ceramide (palmitoyl ceramide, pCer) in bilayers composed basically of dioleoylphosphatidylcholine, sphingomyelin (either C24:1, nSM or C16:0, pSM) and cholesterol. Combination of equimolecular amounts of C24:1 and C16:0 sphingolipids were also studied under the same conditions. Results show that both pCer and nCer are capable of forming segregated gel domains. Force spectroscopy data point to nCer having a lower stiffening effect than pCer, while the presence of nSM reduces the stiffness. DSC reveals Tm reduction by nSM in every case. Furthermore, pSM seems to better accommodate both ceramides in a single phase of intermediate properties, while nSM partial accommodation of ceramides generates different gel phases with higher stiffnesses caused by interceramide cooperation. If both pSM and nSM are present, a clear preference of both ceramides toward pSM is observed. These findings show the ...Continue Reading

References

Jun 12, 1986·Biochimica Et Biophysica Acta·H M McConnellA A Brian
Mar 19, 1993·Science·L M ObeidY A Hannun
Jun 5, 1997·Nature·K Simons, E Ikonen
Jul 27, 2000·Journal of Cellular Physiology·R N KolesnickA Alonso
Nov 7, 2000·Biochimica Et Biophysica Acta·J F Nagle, S Tristram-Nagle
Mar 30, 2001·The Journal of Biological Chemistry·H GrassmeE Gulbins
Apr 5, 2001·The Journal of Biological Chemistry·A CremestiR Kolesnick
Dec 26, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·Heike GrassméErich Gulbins
Jan 18, 2002·The Journal of Biological Chemistry·L Ruth MontesAlicia Alonso
May 15, 2002·The Journal of Biological Chemistry·Leah J SiskindMarco Colombini
Jul 12, 2003·The Journal of Biological Chemistry·F-Xabier ContrerasFélix M Goñi
Aug 4, 2004·EMBO Reports·Anthony H Futerman, Yusuf A Hannun
Sep 10, 2005·Biophysical Journal·Sergi Garcia-ManyesFausto Sanz
Dec 15, 2005·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Erich Gulbins, Pin Lan Li
Mar 28, 2006·Biophysical Journal·Salvatore ChiantiaPetra Schwille
Dec 13, 2006·Biochimica Et Biophysica Acta·Tarek A TahaLina M Obeid
Jun 15, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Salvatore ChiantiaPetra Schwille
Oct 2, 2007·Journal of Molecular Recognition : JMR·Oscar DomènechJordi Hernández-Borrell
Jul 3, 2008·Cancer Research·Joan MonteroJosé C Fernández-Checa
Nov 18, 2008·Histology and Histopathology·C Garcia-RuizJ C Fernández-Checa
Feb 25, 2010·FEBS Letters·Branka Stancevic, Richard Kolesnick
Aug 31, 2010·Journal of the American Chemical Society·Sergi Garcia-ManyesFausto Sanz
Sep 24, 2010·Nature Reviews. Molecular Cell Biology·Kai Simons, Mathias J Gerl
Jun 23, 2011·The Journal of Biological Chemistry·Yusuf A Hannun, Lina M Obeid
Feb 8, 2013·International Journal of Molecular Sciences·Simon J AttwoodZoya Leonenko
Feb 12, 2014·Progress in Lipid Research·Bruno M CastroLiana C Silva
Feb 26, 2014·International Journal of Molecular Sciences·Wenyuan ZhuHuiming Wang
May 6, 2014·Trends in Pharmacological Sciences·Johannes KornhuberErich Gulbins
Feb 1, 2015·Apoptosis : an International Journal on Programmed Cell Death·Elisabeth LangFlorian Lang
Feb 20, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Aritz B García-ArribasFélix M Goñi

❮ Previous
Next ❯

Citations

Apr 6, 2018·Annual Review of Biophysics·Alicia Alonso, Félix M Goñi
Aug 26, 2020·Scientific Reports·Emilio J González-RamírezAlicia Alonso
Oct 21, 2020·Scientific Reports·Igor de la AradaJosé-Luis R Arrondo

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.