PMID: 8583572Mar 1, 1996Paper

Complex formation between PSA isoenzymes and protease inhibitors

The Journal of Urology
J LeinonenU H Stenman

Abstract

We have studied complex formation between the isoenzymes of prostate specific antigen (PSA) and protease inhibitors in vitro. Ion exchange chromatography and hydrophobic interaction chromatography (HIC) were used for rapid separation of PSA isoenzymes from inhibitors and for characterization of the complex formation. Immunofluorometric assays (IFMA) specific for free PSA, the PSA-alpha 1-antichymotrypsin (PSA-ACT) complex and for both of these (total PSA) were used to measure various forms of PSA. Loss of free PSA immunoreactivity was used to estimate complex formation with alpha 2-macroglobulin (A2M) and ACT, which also was measured by PSA-ACT IFMA. Complex formation between PSA and A2M was more rapid than with ACT. After extended incubation, about 75% of PSA reacted with ACT and 85% with A2M. When added to a mixture of ACT and A2M at concentrations corresponding to those in plasma, only 17% of PSA formed a complex with ACT while 17% remained free and 66% was undetectable, indicating complex formation with A2M. After extended incubation of PSA-ACT at 37C, a significant proportion of PSA was released as free active PSA. When A2M was included in the reaction mixture, the loss of PSA-ACT was not accompanied by appearance of free ...Continue Reading

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Citations

Jun 7, 2008·Journal of Biosciences·Abdul WaheedFaizan Ahmad
Jan 3, 1997·Clinica Chimica Acta; International Journal of Clinical Chemistry·C Becker, H Lilja
Jan 11, 2000·Urology·U StenmanJ Leinonen
Jun 1, 1997·European Journal of Clinical Chemistry and Clinical Biochemistry : Journal of the Forum of European Clinical Chemistry Societies·M BarakA Lurie
Aug 6, 2000·Clinical Chemistry and Laboratory Medicine : CCLM·C StephanS A Loening
Aug 25, 2011·Biomarkers in Medicine·Mark R Haythorn, Richard J Ablin
Feb 4, 2016·International Journal of Biological Macromolecules·Paresh B BhanushaliChander P Puri
Jun 11, 2016·International Braz J Urol : Official Journal of the Brazilian Society of Urology·Fatih TarhanOzlem Cakir Madenci
Jul 19, 2012·The Prostate·Lei ZhuHannu Koistinen
Jun 14, 2008·The Journal of Urology·Amanda Beth ReedDipen J Parekh
Dec 22, 1999·British Journal of Cancer·H H HeidtmannR E Kontermann
Aug 10, 2011·Prostate Cancer and Prostatic Diseases·A P CummingW C Duivenvoorden
May 17, 2008·The Journal of Biological Chemistry·Nashmil EmamiEleftherios P Diamandis
Mar 1, 1997·British Journal of Urology·U H Stenman
Jun 18, 2005·Clinical Chemistry·Christina V ObiezuEleftherios P Diamandis
Nov 25, 2003·Clinical Chemistry·Ping WuJari Leinonen
Oct 14, 2006·Current Opinion in Urology·U H StenmanP Wu
Jan 1, 2008·Scandinavian Journal of Clinical and Laboratory Investigation·Rami A MahfouzRose T Daher
Dec 23, 2014·Medicinal Research Reviews·Herve Aloysius, Longqin Hu

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