PMID: 2872210Mar 1, 1986

Complex formation between reduced D-amino acid oxidase and pyridine carboxylates

Journal of Biochemistry
Y NishinaK Shiga


Picolinate binds to a reduced form of D-amino acid oxidase, and the complex formed has a broad absorption band around 600 nm as in the case of the purple intermediate of the enzyme with a substrate. The dissociation constant at 25 degrees C was 35 microns at pH 7.0. The pH dependence (pH 8.3-pH 6.4) of the dissociation constant indicates that one proton is associated with the complex formation, and picolinate protonated at the N atom binds to the reduced enzyme. Resonance Raman spectra of the complex support that picolinate in the complex is a cationic form protonated at the N atom. Nicotinate also binds to the reduced enzyme, but isonicotinate does not.

Related Concepts

D-Amino Acid Dehydrogenase
Raman Optical Activity Spectroscopy
Structure-Activity Relationship
Pyridine, hydrogen iodide
Niacin Manganese (2+) Salt

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