Complex I Controls Mitochondrial and Plasma Membrane Potentials in Nerve Terminals.

Neurochemical Research
Seán M KilbrideGavin P Davey

Abstract

Reductions in the activities of mitochondrial electron transport chain (ETC) enzymes have been implicated in the pathogenesis of numerous chronic neurodegenerative disorders. Maintenance of the mitochondrial membrane potential (Δψm) is a primary function of these enzyme complexes, and is essential for ATP production and neuronal survival. We examined the effects of inhibition of mitochondrial ETC complexes I, II/III, III and IV activities by titrations of respective inhibitors on Δψm in synaptosomal mitochondria. Small perturbations in the activity of complex I, brought about by low concentrations of rotenone (1-50 nM), caused depolarisation of Δψm. Small decreases in complex I activity caused an immediate and partial Δψm depolarisation, whereas inhibition of complex II/III activity by more than 70% with antimycin A was required to affect Δψm. A similarly high threshold of inhibition was found when complex III was inhibited with myxothiazol, and inhibition of complex IV by more than 90% with KCN was required. The plasma membrane potential (Δψp) had a complex I inhibition threshold of 40% whereas complex III and IV had to be inhibited by more than 90% before changes in Δψp were registered. These data indicate that in synaptosome...Continue Reading

References

Aug 1, 1992·Journal of Neurochemistry·S J KishJ N Nobrega
Mar 1, 1990·Journal of Neurochemistry·A H SchapiraC D Marsden
Jun 3, 1989·Lancet·A H SchapiraC D Marsden
Jun 1, 1985·Journal of Neurochemistry·W A BrennanJ R Aprille
Dec 1, 1986·Journal of Neurochemistry·R A Kauppinen, D G Nicholls
Mar 15, 1993·European Journal of Biochemistry·D G Nicholls
Jan 1, 1995·Methods in Enzymology·M ReersL B Chen
Nov 12, 1996·Biochimica Et Biophysica Acta·M ErecińskaI A Silver
May 28, 1998·The Journal of Biological Chemistry·G P DaveyJ B Clark
Sep 23, 1998·Experimental Neurology·M P MattsonJ G Begley
Jan 5, 2000·Physiological Reviews·D G Nicholls, S L Budd
Oct 26, 2000·The Journal of Biological Chemistry·C D BortnerJ A Cidlowski
Dec 2, 2000·Nature Neuroscience·R BetarbetJ T Greenamyre
Oct 23, 2003·Neurochemical Research·David G Nicholls
Dec 15, 2004·Biochemical and Biophysical Research Communications·W Davis Parker, Janice K Parks
Mar 22, 2005·Biochemical Society Symposium·Martin D BrandKarim S Echtay
Oct 6, 2006·American Journal of Physiology. Cell Physiology·Ana Navarro, Alberto Boveris
Dec 7, 2007·Brain Research·W Davis ParkerRussell H Swerdlow
Jun 3, 2008·Biochimica Et Biophysica Acta·Seán M KilbrideGavin P Davey
Feb 6, 2009·The Journal of Biological Chemistry·Jayne E TelfordGavin P Davey
Jan 19, 2010·The Journal of Biological Chemistry·Jayne E TelfordGavin P Davey

❮ Previous
Next ❯

Citations

May 1, 2021·International Journal of Molecular Sciences·Stephanie Cristine Hepp RehfeldtMárcia Inês Goettert

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.