PMID: 9446680Apr 4, 1998Paper

Complex I of Rhodobacter capsulatus and its role in reverted electron transport

Archives of Microbiology
S M HerterG Drews

Abstract

The activities of NAD+-photoreduction and NADH/decyl-ubiquinone reductase in membrane preparations of Rhodobacter capsulatus changed to the same extent under different conditions. These results indicated that NADH:ubiquinone oxidoreductase (complex I) catalyzes the electron transport in the downhill direction (respiratory chain) and in the uphill direction (reverted electron flow). This conclusion was confirmed by the characterization of a complex-I-deficient mutant of R. capsulatus. The mutant was not able to reduce NAD+ in the light. Since this mutant was not able to grow photoautotrophically, we concluded that complex I is the enzyme that catalyzes the reverted electron flow to NAD+ to provide reduction equivalents for CO2 fixation. Complex I is not essential for the reverted electron flow to nitrogenase since the mutant grew under nitrogen-fixing conditions. As shown by immunological means, NuoE, a subunit of complex I from R. capsulatus having an extended C-terminus, was modified depending on the nitrogen source present in the growth medium. When the organism used N2 instead of NH4+, a smaller NuoE polypeptide was synthesized. The complex-I-deficient mutant was not able to modify NuoE. The function of the modification is d...Continue Reading

Citations

Nov 28, 2007·Journal of Bacteriology·Gloria HerrmannWolfgang Buckel
Feb 19, 2011·International Journal of Endocrinology·Noormuhammad Oosman AbbasakoorDermot O'Toole
Jul 23, 2011·BMC Systems Biology·Saheed ImamTimothy J Donohue
Jan 1, 2014·Photosynthesis Research·André Verméglio, Pierre Joliot
May 22, 2010·Molecular Microbiology·Federico E Rey, Caroline S Harwood
Feb 3, 2016·Journal of Bacteriology·Melanie A SperoTimothy J Donohue
Apr 29, 2006·Journal of Molecular Microbiology and Biotechnology·Clara D BoiangiuWolfgang Buckel
Feb 24, 2019·Archives of Microbiology·Nilüfer Afsar ErkalMeral Yücel
Jun 6, 2021·Bioresource Technology·Ferdinand SchmidJohannes Gescher
Jul 30, 2021·Biotechnology and Applied Biochemistry·S Balaji

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