PMID: 6163871Feb 1, 1981Paper

Complex of simian virus 40 large-T antigen and host 53,000-molecular-weight protein in monkey cells

Journal of Virology
E HarlowL V Crawford

Abstract

Mouse cells transformed by simian virus 40 (SV40) have been shown to contain a complex of the virus-coded large-T antigen with a host 53,000-molecular-weight (53K) protein. Initial attempts to detect a similar complex in lytically infected cells were unsuccessful, and it therefore seemed that the complex might be peculiar to transformed or abortively transformed nonpermissive cells. Immunoprecipitation of [32P]phosphate-labeled extracts of SV40-infected CV-1 African green monkey kidney cells with antibodies specific for large-T or the 53K protein revealed that the large-T-53K protein complex was formed during lytic infections. Only a minor fraction of the large-T present was associated with 53K protein, and large-T and the 53K host protein cosedimented during centrifugation through sucrose gradients. We used monospecific sera and monoclonal antibodies to study the rate of synthesis and phosphorylation of the 53K protein during lytic infections. Infection of CV-1 cells with SV40 increased the rate of synthesis of the 53K protein fivefold over that in mock-infected cells. At the same time, the rate of phosphorylation of the 53K protein increased more than 30-fold compared with control cultures. Monkey cells transformed by UV-irra...Continue Reading

References

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Oct 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·P GaudrayF Cuzin
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Citations

Oct 22, 1985·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·D P LaneS Mole
Jan 1, 1982·CRC Critical Reviews in Biochemistry·B Schaffhausen
Feb 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·M NevelsT Dobner
Nov 15, 1996·The Journal of Biological Chemistry·K K KimL J Field
Apr 1, 1992·Journal of Cellular Physiology·M M WitteR E Scott
Apr 1, 1983·European Journal of Immunology·S Swaminathan, L R Gooding
Nov 15, 1983·International Journal of Cancer. Journal International Du Cancer·K LevitskyD T Simmons
Mar 1, 1990·Journal of Cellular Physiology·D N EstervigR E Scott
Apr 3, 1998·Journal of Virology·M E Martin, A J Berk
Jan 9, 1999·Journal of Virology·M NevelsT Dobner
Jun 21, 2001·Molecular Cell Biology Research Communications : MCBRC·A J Darmon, P S Jat
Feb 1, 1983·Journal of Virology·M Montenarh, R Henning
Aug 1, 1986·Journal of Virology·J W YewdellD P Lane
Jun 1, 1989·Journal of Virology·D N EstervigR E Scott
Dec 1, 1988·Journal of Virology·S KraissM Montenarh

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