Complex pattern of insulin-like growth factor binding protein expression in primary rat osteoblast enriched cultures: regulation by prostaglandin E2, growth hormone, and the insulin-like growth factors

Journal of Cellular Physiology
T L McCarthyE Canalis

Abstract

Primary osteoblast-enriched (Ob) cultures from fetal rat bone synthesize insulin-like growth factor (IGF) I and IGF-II, which each enhance Ob function. While a number of agents modulate IGF-I production, IGF-II is constitutively expressed in this culture model. Independent of their expression, however, the activity of the IGFs can be modified by a small group of proteins termed IGF binding proteins (IGFBPs), but little is known about the regulation of individual IGFBPs that are synthesized by Ob cells. Northern blot analysis revealed that serum-deprived primary rat Ob cells express transcripts encoding IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-5, and IGFBP-6, but undetectable levels of IGFBP-1 transcripts. Western ligand blots of Ob culture medium probed with 125I-IGF-I or 125I-IGF-II showed predominant IGFBPs migrating at 30/32 kDa, with minor bands at 24 and 38-47 kDa. Western antibody analysis identified IGFBP-2 and IGFBP-5 within the 30/32 kDa complex, while gel mobility shift on SDS-PAGE following deglycosylation determined that IGFBP-3 comprised the 38-47 kDa complex. By Northern analysis, 6 h treatment with prostaglandin E2 (PGE2), growth hormone (hGH), IGF-I, or IGF-II revealed a complex pattern of regulatory effects on steady-s...Continue Reading

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Citations

Jul 1, 1997·Journal of Cellular Biochemistry·B Gabbitas, E Canalis
Sep 1, 1996·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·B A WatkinsM F Seifert
Nov 1, 1994·Journal of Cellular Biochemistry·A M DelanyE Canalis
Jan 1, 1997·Archives of Orthopaedic and Trauma Surgery·H KienapfelP Griss
Jan 1, 1996·Baillière's Clinical Endocrinology and Metabolism·D LeRoith
Nov 9, 2002·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·Dan Jia, Johan N M Heersche
Apr 26, 2008·Endocrine Reviews·Andrea GiustinaErnesto Canalis
Jun 4, 1998·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M ThorénS Mohan
Jul 14, 1998·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·J SvenssonB A Bengtsson
Jul 15, 1999·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Y LiB A Watkins
May 10, 2000·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M KanataniK Chihara
Jun 2, 2009·Expert Opinion on Investigational Drugs·Gui Tong TranEleftherios Tsiridis
Jul 6, 2010·Progress in Lipid Research·M C KrugerH Weiler
Mar 20, 1999·Baillière's Clinical Endocrinology and Metabolism·G Johannsson, C Ohlsson
Mar 10, 2007·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·Christer EhrnborgThord Rosén
Mar 27, 2007·Injury·Eleftherios TsiridisPeter Giannoudis
Feb 24, 2007·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·Aditi Mukherjee, Peter Rotwein
Jul 3, 2004·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·D DurantE Canalis
Nov 3, 2004·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·Thor Ueland
Apr 18, 2006·Injury·Rozalia DimitriouPeter V Giannoudis
Mar 27, 2002·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·K McQueeney, C N Dealy
Sep 30, 1999·The Veterinary Clinics of North America. Small Animal Practice·D L Millis
Apr 24, 2018·Endocrine Reviews·Gherardo MazziottiAndrea Giustina
Aug 1, 1997·Journal of Cellular Physiology·B Gabbitas, E Canalis

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