Complex regulation of store-operated Ca2+ entry pathway by PKC-epsilon in vascular SMCs.

American Journal of Physiology. Cell Physiology
Tarik SmaniV M Bolotina

Abstract

The role of PKC in the regulation of store-operated Ca2+ entry (SOCE) is rather controversial. Here, we used Ca2+-imaging, biochemical, pharmacological, and molecular techniques to test if Ca2+-independent PLA2beta (iPLA2beta), one of the transducers of the signal from depleted stores to plasma membrane channels, may be a target for the complex regulation of SOCE by PKC and diacylglycerol (DAG) in rabbit aortic smooth muscle cells (SMCs). We found that the inhibition of PKC with chelerythrine resulted in significant inhibition of thapsigargin (TG)-induced SOCE in proliferating SMCs. Activation of PKC by the diacylglycerol analog 1-oleoyl-2-acetyl-sn-glycerol (OAG) caused a significant depletion of intracellular Ca2+ stores and triggered Ca2+ influx that was similar to TG-induced SOCE. OAG and TG both produced a PKC-dependent activation of iPLA2beta and Ca2+ entry that were absent in SMCs in which iPLA2beta was inhibited by a specific chiral enantiomer of bromoenol lactone (S-BEL). Moreover, we found that PKC regulates TG- and OAG-induced Ca2+ entry only in proliferating SMCs, which correlates with the expression of the specific PKC-epsilon isoform. Molecular downregulation of PKC-epsilon impaired TG- and OAG-induced Ca2+ influx...Continue Reading

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Citations

Jun 12, 2012·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Yadong GaoJiong Yang
Nov 12, 2014·Cellular and Molecular Bioengineering·Allison M AndrewsKenneth A Barbee
Jan 10, 2012·Arteriosclerosis, Thrombosis, and Vascular Biology·Alejandro Domínguez-RodríguezTarik Smani
Aug 11, 2011·The Journal of Pharmacy and Pharmacology·Patrícia A Fonseca-MagalhãesAlice M C Martins
Mar 5, 2011·Basic & Clinical Pharmacology & Toxicology·Jong-Khing HuangChung-Ren Jan
Aug 19, 2016·The Journal of Biological Chemistry·Javier Ávila-MedinaTarik Smani

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