Complex regulation of the immunoglobulin mu heavy-chain gene enhancer: microB, a new determinant of enhancer function.

Molecular and Cellular Biology
B NelsenR Sen

Abstract

The B-lymphocyte-specific activity of the immunoglobulin mu heavy-chain gene enhancer has been attributed to the octamer motif (ATTTGCAT) present within the enhancer that binds a B-cell-specific factor designated NF-A2/OTF-2. However, significant residual enhancer activity even after deletion of this element has suggested the presence of a second critical functional determinant. We have used deletion and mutational analyses to define an element, microB (TTTGGGGAA), that is essential for B-cell-specific enhancer activity in S194 myeloma cells in the absence of the octamer. Transfection analysis in a panel of lymphoid cell lines suggests that the presence of either microB or octamer leads to considerable enhancer activity in cell lines representing later stages of B-cell differentiation, whereas both elements are needed for function in cell lines representing earlier stages. Furthermore, in contrast to the results in pre-B-cell lines, both microB and octamer elements function independently in certain T-cell lines in which the mu enhancer is active.

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Citations

Oct 18, 1996·The Journal of Biological Chemistry·Y AkbaraliT A Libermann
Oct 21, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jun-Ichi HikimaGregory W Warr
Oct 17, 1998·Molecular and Cellular Biology·W DangR Sen
Oct 27, 1995·The Journal of Biological Chemistry·Y H LinJ E Niederhuber
Jul 1, 1997·Molecular and Cellular Biology·B S NikolajczykR Sen
Nov 1, 1993·Molecular and Cellular Biology·R R RiveraC Murre
Oct 1, 1993·Molecular and Cellular Biology·T A Libermann, D Baltimore
Apr 1, 1994·Current Opinion in Immunology·J Hagman, R Grosschedl
Jun 1, 1994·Immunity·L VenkataramanR Sen

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