Complex signals in the genomic 3' nontranslated region of bovine viral diarrhea virus coordinate translation and replication of the viral RNA

RNA
Olaf IskenSven-Erik Behrens

Abstract

The genomes of positive-strand RNA viruses strongly resemble cellular mRNAs. However, besides operating as a messenger to generate the virus-encoded proteins, the viral RNA serves also as a template during replication. A central issue of the viral life cycle, the coordination of protein and RNA synthesis, is yet poorly understood. Examining bovine viral diarrhea virus (BVDV), we report here on the role of the variable 3'V portion of the viral 3' nontranslated region (3'NTR). Genetic studies and structure probing revealed that 3'V represents a complex RNA motif that is composed of synergistically acting sequence and structure elements. Correct formation of the 3'V motif was shown to be an important determinant of the viral RNA replication process. Most interestingly, we found that a proper conformation of 3'V is required for accurate termination of translation at the stop-codon of the viral open reading frame and that efficient termination of translation is essential for efficient replication of the viral RNA. Within the viral 3'NTR, the complex 3'V motif constitutes also the binding site of recently characterized cellular host factors, the so-called NFAR proteins. Considering that the NFAR proteins associate also with the 5'NTR...Continue Reading

References

May 12, 1971·Nature: New Biology·D Kolakofsky, C Weissmann
Aug 8, 1998·Genes & Development·A V Gamarnik, R Andino
Mar 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·M YanagiJ Bukh
Oct 3, 1999·Virus Research·V I AgolE Wimmer
Aug 31, 2000·Annual Review of Biochemistry·A J HerrR F Gesteland

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Citations

Aug 9, 2007·RNA·Olaf IskenSven-Erik Behrens
Jul 17, 2009·RNA·Cristina Romero-López, Alfredo Berzal-Herranz
Jul 27, 2012·Nucleic Acids Research·Urszula M Wolkowicz, Atlanta G Cook
Aug 14, 2012·Biologicals : Journal of the International Association of Biological Standardization·John D Neill
Feb 10, 2012·Current Opinion in Virology·Lucas C Reineke, Richard E Lloyd
Sep 29, 2009·Biochimica Et Biophysica Acta·Ying LiuAniko V Paul
Jan 17, 2015·The Journal of General Virology·Chun ShengMing Xiao
Apr 25, 2014·The Journal of General Virology·Mandy GebauerSven-Erik Behrens
Jan 8, 2010·The Journal of General Virology·Martina BarothSven-Erik Behrens
Oct 8, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Sujie NiWeijian Guo
Mar 30, 2011·RNA Biology·Dorota Piekna-Przybylska, Robert A Bambara
Apr 21, 2011·RNA Biology·Zhenghe Li, Peter D Nagy
Aug 19, 2008·Virus Research·Jan ZollWillem J G Melchers
Dec 3, 2014·Biochimie·Claudia PatiñoSilvio Urcuqui-Inchima
Jan 30, 2013·PLoS Neglected Tropical Diseases·Kali D Saxton-ShawAnn M Powers
Mar 28, 2009·Virology·Carlos Sandoval-Jaime, Ana Lorena Gutiérrez-Escolano
Jun 24, 2004·Progress in Nucleic Acid Research and Molecular Biology·K Andrew White, Peter D Nagy
Aug 10, 2018·Wiley Interdisciplinary Reviews. RNA·Manuel Garcia-MorenoAlfredo Castello

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