Complex structures of the abscisic acid receptor PYL3/RCAR13 reveal a unique regulatory mechanism

Structure
Xingliang ZhangZhongzhou Chen

Abstract

Abscisic acid (ABA) controls many physiological processes and mediates adaptive responses to abiotic stresses. The ABA signaling mechanisms for abscisic acid receptors PYR/PYL/RCAR (PYLs) were reported. However, it remains unclear whether the molecular mechanisms are suitable for other PYLs. Here, complex structures of PYL3 with (+)-ABA, pyrabactin and HAB1 are reported. An unexpected trans-homodimer intermediate observed in the crystal is confirmed in solution. ABA-bound PYL3 greatly promotes the generation of monomeric PYL3, which can excessively increase the efficiency of inhibiting PP2Cs. Structure-guided biochemical experiments show that Ser195 accounts for the key intermediate. Interestingly, pyrabactin binds to PYL3 in a distinct nonproductive mode with gate closure, which sheds light on the design of agonists and antagonists for abscisic acid receptors. According to different conformations of ligand-bound PYLs, the PYLs family can be divided into three subclasses, among which the trans-dimeric subclass, represented by PYL3, reveals a distinct regulatory mechanism.

References

Feb 1, 1996·Journal of Molecular Graphics·R KoradiK Wüthrich
Apr 15, 2000·The Journal of Biological Chemistry·S BarbirzM O Glocker
Jun 5, 2002·The Plant Cell·Ruth R FinkelsteinChristopher D Rock
Dec 12, 2002·Mass Spectrometry Reviews·Jeffrey J GormanJames J Pitt
Aug 8, 2007·Journal of Molecular Biology·Evgeny Krissinel, Kim Henrick
Sep 27, 2008·Bioinformatics·L HolmA Schenkel
Oct 27, 2009·Nature·Ken-Ichi MiyazonoMasaru Tanokura
Nov 7, 2009·Nature Structural & Molecular Biology·Ping YinNieng Yan
Nov 10, 2009·Nature·Julia SantiagoJosé Antonio Márquez
Nov 20, 2009·Nature·Hiroaki FujiiJian-Kang Zhu
Nov 26, 2009·Science·Noriyuki NishimuraElizabeth D Getzoff
Jan 27, 2010·Cell Research·Lin YuZhongzhou Chen
Mar 3, 2010·Annual Review of Plant Biology·Sean R CutlerSuzanne R Abrams
May 25, 2010·Trends in Plant Science·Agepati S RaghavendraErwin Grill
Jun 18, 2010·The Journal of Biological Chemistry·Qi HaoNieng Yan
Aug 24, 2010·Nature Structural & Molecular Biology·Francis C PetersonBrian F Volkman
Aug 24, 2010·Nature Structural & Molecular Biology·Karsten MelcherH Eric Xu
Aug 19, 2011·The EMBO Journal·Florine DupeuxJosé Antonio Márquez
Jan 1, 1997·Methods in Enzymology·Zbyszek Otwinowski, Wladek Minor

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Citations

May 6, 2014·Nature Chemical Biology·Jun TakeuchiYasushi Todoroki
Jul 3, 2013·Proceedings of the National Academy of Sciences of the United States of America·Masanori OkamotoSean R Cutler
Nov 26, 2013·The Arabidopsis Book·Ruth Finkelstein
May 3, 2014·Acta Pharmacologica Sinica·Ley Moy NgH Eric Xu
Apr 17, 2014·ELife·Alexander M JonesWolf B Frommer
Jul 5, 2013·PLoS Computational Biology·Lyudmyla DoroshMaria Stepanova
Aug 13, 2013·Plant Molecular Biology·Ge BaiJian-Kang Zhu
Sep 19, 2015·Critical Reviews in Biotechnology·Amarjeet SinghGirdhar K Pandey
Dec 26, 2012·Trends in Plant Science·Takuya MiyakawaMasaru Tanokura
Nov 28, 2015·Bioorganic & Medicinal Chemistry·Jonathan D M HelanderSean R Cutler
Mar 22, 2014·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Masahiro NakagawaToshio Hakoshima
Mar 10, 2015·Frontiers in Plant Science·Xing L ZhangZhong Z Chen
May 23, 2013·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Xingliang ZhangGuoqiang Wang
Mar 9, 2017·Scientific Reports·Xiaoqiang HanZhaohai Qin
Jul 15, 2017·ACS Chemical Biology·Josie A SilvaroliMarcin Golczak
Sep 7, 2017·Proceedings of the National Academy of Sciences of the United States of America·Stefanie V TischerErwin Grill
Oct 27, 2017·Scientific Reports·Zhizhong RenJian-Kang Zhu
Jul 27, 2017·The Plant Journal : for Cell and Molecular Biology·Michael PapacekErwin Grill
Apr 4, 2019·Plant Molecular Biology·Seungsu HanSangho Lee
Aug 24, 2018·Nature Communications·Wenli TianZhongzhou Chen
Mar 20, 2021·FEBS Open Bio·Yutao WangXingliang Zhang
Jun 3, 2021·Plants·Rafael Ruiz-PartidaJorge Lozano-Juste

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