Complexation of bioreducible cationic polymers with gold nanoparticles for improving stability in serum and application on nonviral gene delivery

ACS Applied Materials & Interfaces
Chun-Chiao Chuang, Chien-Wen Chang

Abstract

Widespread applications of conventional polymeric gene carriers are greatly hampered by their inefficient transfection performance under serum-containing environment. Aiming to overcome this limitation, we propose a bioreducible polyethylenimine-gold nanocomplex system (SSPEI-Au NC), which can be prepared by a simple layer-by-layer (LBL) assembly procedure. SSPEI-Au NC contains sequentially deposited layers of bioreducible polyethylenimine (SSPEI) and poly(γ-glutamic acid) (γ-PGA) for efficient binding and delivery of plasmid DNA (pDNA). SSPEI-Au NC was characterized for various physicochemical properties, including: UV-vis spectra, TEM imaging, hydrodynamic size, and pDNA binding ability. The SSPEI-Au NC were efficiently uptaken by mammalian cells as observed using dark-field microscopy. Comparing to nondegradable PEI25k, the bioreducible SSPEI-Au NC exhibited superior transfection capability under serum-containing condition while causing lower cytotoxicity on mammalian cell lines. The effect of serum on SSPEI-Au NC dispersity was studied using UV-vis spectrometry and the results suggest that serum-assisted colloidal stability of SSPEI-Au NC contributed to its serum-resistant transfection.

References

Mar 22, 2001·Advanced Drug Delivery Reviews·C W Pouton, L W Seymour
Dec 9, 2003·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Ferry VerbaanChristien Oussoren
Sep 21, 2006·Bioconjugate Chemistry·Lane V ChristensenJan Feijen
Aug 4, 2007·Lasers in Medical Science·Xiaohua HuangMostafa A El-Sayed
Jan 31, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Annie DorrisR Bruce Lennox
Dec 17, 2009·Journal of Controlled Release : Official Journal of the Controlled Release Society·Dakrong PissuwanMichael B Cortie
Sep 8, 2010·Advanced Materials·Brian P TimkoDaniel S Kohane
Jul 14, 2011·Nucleic Acid Therapeutics·Kaushik SinghaWon Jong Kim
Nov 1, 2011·Journal of Controlled Release : Official Journal of the Controlled Release Society·Wei XiaXiaohu Zhao
Nov 1, 2012·Biotechnology Advances·Anil KumarXing-Jie Liang
Apr 9, 2013·Journal of Controlled Release : Official Journal of the Controlled Release Society·Wen-Hsuan ChiangHsin-Cheng Chiu
Nov 30, 2013·Biomaterials·Elizabeth R FigueroaRebekah A Drezek
Apr 22, 2014·Journal of Controlled Release : Official Journal of the Controlled Release Society·Young Sook Lee, Sung Wan Kim
Oct 6, 2014·Nature Materials·Young-Wook WonYong-Hee Kim
Jul 7, 2014·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Xiaofang ZhangZhaozhong Jiang

❮ Previous
Next ❯

Citations

Jan 14, 2016·Expert Opinion on Biological Therapy·Jessica Pahle, Wolfgang Walther
Oct 1, 2015·ACS Applied Materials & Interfaces·Yajie ZhangFude Feng
Sep 30, 2016·Chemistry, an Asian Journal·Mariano Ortega-MuñozFrancisco Santoyo-Gonzalez
Mar 9, 2021·Wiley Interdisciplinary Reviews. RNA·Mingyuan ZouJian Xu
Jul 2, 2019·Acta Pharmaceutica : a Quarterly Journal of Croatian Pharmaceutical Society and Slovenian Pharmaceutical Society, Dealing with All Branches of Pharmacy and Allied Sciences·Londiwe Simphiwe MbathaMoganavelli Singh
May 4, 2021·Chemical Reviews·Ramya KumarTheresa M Reineke
May 5, 2021·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Rih-Yang HuangChien-Wen Chang
Jul 3, 2021·Pharmaceutics·Londiwe Simphiwe MbathaMoganavelli Singh
Sep 18, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Raffaella BucciSara Pellegrino

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.