PMID: 9650559Jul 3, 1998Paper

Complexes between urokinase-type plasminogen activator and its receptor in blood as determined by enzyme-linked immunosorbent assay

International Journal of Cancer. Journal International Du Cancer
H de WitteT Benraad

Abstract

Complexes between urokinase-type plasminogen activator (uPA) and its receptor (uPAR) were assessed in plasma and serum from 39 breast cancer patients and from 20 healthy individuals, applying a recently developed enzyme-linked immunosorbent assay (ELISA) for the analysis of these complexes in tumor tissue extracts. The assay is based on a combination of rabbit polyclonal anti-uPA antibodies for catching and a mouse anti-uPAR monoclonal antibody (MAb) for detection. The specificity of the assessment of uPA:uPAR complexes was verified by simultaneous analysis of the individual blood samples in corresponding non-sense ELISA formats, in which either the anti-uPA catching antibody or the anti-uPAR detecting antibody was substituted with an irrelevant antibody. Assessment of native uPA:uPAR complexes was ascertained by demonstrating the absence of any de novo formation of uPA:uPAR complexes in plasma and serum during the sample incubation step in the ELISA, as verified by the use of a peptide antagonist for uPAR. Plasma and serum samples contained almost identical levels of uPA:uPAR complexes. The levels of uPA:uPAR complexes were found to be significantly lower in serum from breast cancer patients compared to the serum of healthy do...Continue Reading

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Citations

Oct 15, 2013·Journal of Critical Care·Katia DonadelloJean-Louis Vincent
Nov 6, 2015·The New England Journal of Medicine·Salim S HayekJochen Reiser
Jan 21, 2015·Respiratory Physiology & Neurobiology·A Wrotek, T Jackowska
Sep 4, 2016·Talanta·Xiaolei ZhouMingdong Huang
Oct 24, 2007·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Lyndsay HarrisUNKNOWN American Society of Clinical Oncology
Apr 12, 2011·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·T MölkänenA Järvinen
Sep 4, 2004·Clinical Chemistry·Timo PiironenGunilla Høyer-Hansen
May 10, 2012·Current Opinion in Nephrology and Hypertension·Jochen ReiserJames Tumlin
Sep 24, 2020·Journal of the American Society of Nephrology : JASN·Tariq U AzamUNKNOWN International Study of Inflammation in COVID-19
Jun 21, 2020·Drug Discovery Today·Daniele D'AlonzoVincenzo Pavone

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