Complexes of aspartate aminotransferase with hydroxylamine derivatives: spectral studies in solution and in the crystalline state

Biochimie
L T DelbaereJ N Jansonius

Abstract

Hydroxylamine and its derivatives of general formula H2NOR react with aldehydes and aldimines to produce oximes. If R corresponds to the side chain of a natural amino acid, such compounds can be thought of as analogs of the corresponding amino acids, lacking the alpha-carboxylate group. Oximes formed between such compounds and pyridoxal phosphate in the active site of aspartate amino-transferase mimic external aldimine intermediates that occur during catalysis by this enzyme. The properties of oxime derivatives of mitochondrial aspartate aminotransferase with hydroxylamine and 6 compounds H2NOR were studied by absorption spectroscopy and circular dichroism in solution and by linear dichroism in crystals. Stable oximes, absorbing at lambda max congruent to 380 nm and exhibiting a negative Cotton effect, were obtained with the carboxylate-containing compounds. The oximes formed with carboxylate-free compounds showed somewhat different properties and stability. With H-Tyr a stable complex absorbing at lambda max congruent to 370 nm rather than at 380 nm, was obtained, H-Ala and H-Phe produced unstable oximes with the initial absorption band at lambda max congruent to 380 nm that was gradually replaced by a band at lambda max congr...Continue Reading

References

Jun 1, 1978·The Biochemical Journal·R A John, A Charteris
Jan 1, 1976·Annual Review of Biophysics and Bioengineering·J Hofrichter, W A Eaton
Jan 1, 1966·Biochemical Pharmacology·J P DaVanzoM Daugherty
Apr 30, 1966·Nature·M Y Karpeisky, V I Ivanov
Apr 15, 1984·Journal of Molecular Biology·J F KirschP Christen
Aug 15, 1984·Archives of Biochemistry and Biophysics·R P RaunioW T Jenkins
Feb 1, 1963·Biochemical Pharmacology·E ROBERTS, D G SIMONSEN

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Citations

Mar 15, 1996·European Journal of Biochemistry·Z Marković-HousleyJ N Jansonius
Nov 26, 2015·Acta Crystallographica. Section E, Crystallographic Communications·Isao Fujii
Mar 1, 2019·Water Science and Technology : a Journal of the International Association on Water Pollution Research·Mona M NaimAhmed A El-Shafei

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