Complexing of bacterial lipopolysaccharide with lung surfactant.

Infection and Immunity
K A BrogdenH D Lehmkuhl

Abstract

Lipopolysaccharides (LPS) from Escherichia coli, Salmonella typhi, Klebsiella pneumoniae, Serratia marcescens, or Pseudomonas aeruginosa were mixed with pulmonary surfactant to investigate their in vitro interaction. After 6 h of incubation at 37 degrees C, LPS-surfactant mixtures were examined by sucrose density gradient centrifugation. The E. coli LPS-surfactant mixture was examined by immunoelectron microscopy with protein A-colloidal gold. The binding that occurred between LPS and the surfactant vesicles resulted in a complex with a density higher than the density of the surfactant alone. The protein A-colloidal gold identified LPS in the LPS-surfactant complexes. The toxicity of E. coli LPS was enhanced by complexing with the surfactant when compared with the intraperitoneal injection into CF1 mice, even at a 64:1 ratio of surfactant to LPS. The complexing of LPS and surfactant in the lung may alter the physiologic properties of surfactant that contribute to the physiopathological changes observed with some types of pneumonia.

References

Nov 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·C GalanosW Reutter
Dec 1, 1978·The Journal of Clinical Investigation·R J Ulevitch, A R Johnston
May 1, 1979·The Journal of Clinical Investigation·R K AlbertJ Butler
Apr 14, 1967·Journal of Molecular Biology·J W ShandsK Nath
Dec 1, 1974·Proceedings of the Society for Experimental Biology and Medicine·S M BessaR L Goodale
Mar 16, 1973·Biochimica Et Biophysica Acta·D A BenedettoD O Shah
Jul 1, 1970·Analytical Biochemistry·A Chalvardjian, E Rudnicki
Jan 1, 1984·Experimental Lung Research·K A BrogdenH D Lehmkuhl
Jul 1, 1984·Reviews of Infectious Diseases·Y Takeuchi, H Nikaido
Feb 1, 1981·The Journal of Clinical Investigation·A JobeJ Padbury
Jan 7, 1983·Biochimica Et Biophysica Acta·M W MagoonJ A Clements
Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER
Jan 1, 1964·Annals of Internal Medicine·A I SUTNICK, L A SOLOFF

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Citations

May 1, 1991·Antonie van Leeuwenhoek·K A Brogden
May 29, 1998·American Journal of Respiratory and Critical Care Medicine·H FehrenbachJ Richter
Sep 27, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Maros KolomaznikAndrea Calkovska
Sep 1, 1994·European Journal of Clinical Investigation·U PisonS Pietschmann
Jan 1, 1991·British Journal of Industrial Medicine·A J DeLuccaN M Morris
Nov 11, 1992·British Journal of Industrial Medicine·A J De LuccaA D French
Jun 5, 2020·Lipids in Health and Disease·Christina W AgudeloItsaso Garcia-Arcos
Aug 6, 2010·Veterinary Pathology·K SinghA W Confer
Jul 7, 2018·International Journal of Molecular Sciences·Maros KolomaznikAndrea Calkovska
Feb 12, 2015·Physiological Research·M KolomaznikA Calkovska
Jan 1, 1988·Electron Microscopy Reviews·K A Brogden, M Phillips
Dec 31, 2010·Biophysical Journal·Olga CañadasCristina Casals

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